Optical mapping approaches to cardiac electrophysiological functions.

Optical mapping approaches to cardiac electrophysiological functions.
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心脏电生理功能的光学映射方法。

DOI:
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发表时间:
2001
期刊:
The Japanese Journal of Physiology
影响因子:
--
通讯作者:
K. Kamino
K. Kamino
中科院分区:
--
文献类型:
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作者:
T. Sakai;K. Kamino

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最近,采用快速电压敏感染料监测膜电位的光学方法已被引入,作为研究心脏电功能的有力工具。与更传统的电生理学技术相比,这些方法具有两个主要优点。一是可以从微电极刺穿无法到达的非常小的细胞进行光学记录,二是可以同时监测制剂的多个位点/区域以提供电活动的空间分辨图。前者使得记录早期胚胎预收缩心脏中的自发电活动成为可能,后者已用于绘制心脏组织中电活动的传播模式。本文综述了脊椎动物心脏电生理功能的光学研究。
Recently, optical methods for monitoring membrane potential with fast voltage-sensitive dyes have been introduced as a powerful tool for studying cardiac electrical functions. These methods offer two principal advantages over more conventional electrophysiological techniques. One is that optical recordings may be made from very small cells that are inaccessible to microelectrode impalement, and the other is that multiple sites/regions of a preparation can be monitored simultaneously to provide spatially resolved mapping of electrical activity. The former has made it possible to record spontaneous electrical activities in early embryonic precontractile hearts, and the latter has been applied for mapping of the propagation patterns of electrical activities in the cardiac tissue. In this article, optical studies of the electrophysiological function of the vertebrate heart are reviewed.
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