Mutation of a novel ABC transporter gene is responsible for the failure to incorporate uric acid in the epidermis of ok mutants of the silkworm, Bombyx mori
Mutation of a novel ABC transporter gene is responsible for the failure to incorporate uric acid in the epidermis of ok mutants of the silkworm, Bombyx mori
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DOI：
10.1016/j.ibmb.2013.03.011
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发表时间：
2013-07-01
影响因子：
3.8
通讯作者：
Shimada, Toru
中科院分区：
文献类型：
--
作者：
Wang, Lingyan;Kiuchi, Takashi;Shimada, Toru
家蚕的OK突变体，EscherichiMod，由于不能将尿酸掺入表皮细胞中而表现出高度半透明的幼虫皮肤。在这里，我们报告的确定的基因负责的ok突变的定位克隆和RNAi实验。在两个独立的ok突变株中，我们发现了一个49 bp的缺失和233 bp的重复，分别在一个新的基因，Bm-ok，它编码一个半型ABC转运蛋白，其中每个结果在每个突变体中的截短蛋白的翻译的mRNA。虽然Bm-ok序列与果蝇中的白色、猩红色和棕色转运蛋白基因同源，但在鳞翅目、半翅目和半翅目昆虫基因组中发现的新的直系同源物将其鉴定为转运蛋白新的独特亚家族的成员。Bm-ok的胚胎RNAi表明，Bm-ok的抑制导致一龄家蚕幼虫的半透明表型。我们讨论了Bm-ok与另一种半型ABC转运蛋白Bmwh 3形成异二聚体，并在家蚕表皮中充当尿酸转运蛋白的可能性。(C)2013爱思唯尔有限公司保留所有权利。
ok mutants of the silkworm, Bombyx mod, exhibit highly translucent larval skin resulting from the inability to incorporate uric acid into the epidermal cells. Here we report the identification of a gene responsible for the ok mutation using positional cloning and RNAi experiments. In two independent ok mutant strains, we found a 49-bp deletion and a 233-bp duplication, respectively, in mRNAs of a novel gene, Bm-ok, which encodes a half-type ABC transporter, each of which results in translation of a truncated protein in each mutant. Although the Bm-ok sequence was homologous to well-known transporter genes, white, scarlet, and brown in Drosophila, the discovery of novel orthologs in the genomes of lepidopteran, hymenopteran, and hemipteran insects identifies it as a member of a new distinct subfamily of transporters. Embryonic RNAi of Bm-ok demonstrated that repression of Bm-ok causes a translucent phenotype in the first-instar silkworm larva. We discuss the possibility that Bm-ok forms a heterodimer with another half-type ABC transporter, Bmwh3, and acts as a uric acid transporter in the silkworm epidermis. (C) 2013 Elsevier Ltd. All rights reserved.