Physicochemical, antioxidant, and antimicrobial properties of chitooligosaccharides produced using three different enzyme treatments
Physicochemical, antioxidant, and antimicrobial properties of chitooligosaccharides produced using three different enzyme treatments
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DOI：
10.1016/j.fbio.2017.03.004
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发表时间：
2017-06-01
期刊：
影响因子：
5.2
通讯作者：
Regenstein, Joe M.
中科院分区：
文献类型：
--
作者：
Laokuldilok, Thunnop;Potivas, Thipwimon;Regenstein, Joe M.
本研究的目的是研究三种非底物专一性酶在壳寡糖(COS)生产中的效率，并检测不同平均相对分子质量(MW)的COS的抗氧化和抗菌性能。用溶菌酶、木瓜酶或纤维素酶(0.003%w/w)分别对脱乙酰度分别为80和90的壳聚糖进行0-16h的水解液处理，结果表明，木瓜酶对脱乙酰度为80和90的壳聚糖的降解率最高。DD90经木瓜蛋白酶酶解16h后，平均相对分子质量为4.3 kDa，符合COS的要求(
The objectives of this study were to investigate the efficiency of three non-substrate specific enzymes on chitooligosaccharides (COS) production and to examine the antioxidant and antimicrobial properties of different average molecular weight (MW) COS. Two chitosans with 80 and 90 degrees of deacetylation (DD) were hydrolyzed using lysozyme, papain, or cellulase (0.003% w/w) for 0-16 h. Papain showed the highest MW reduction of chitosan DD90. After 16 h of DD90 hydrolysis with papain, the average MW was 4.3 kDa which meets the requirement to be a COS (