Mycoplasma contamination in fish cell lines: an evaluation of detection by differential incorporation of 3H‐uridine and 14C‐uracil
Mycoplasma contamination in fish cell lines: an evaluation of detection by differential incorporation of 3H‐uridine and 14C‐uracil
复制标题
鱼细胞系中的支原体污染：3H-尿苷和 14C-尿嘧啶差异掺入检测的评估
DOI：
10.1111/j.1365-2761.1986.tb00990.x
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发表时间：
1986
影响因子：
2.5
通讯作者：
F. Hetrick
中科院分区：
文献类型：
--
作者：
C. Schultz;B. Lidgerding;P. McAllister;F. Hetrick
.采用尿嘧啶和尿嘧啶差异掺入法检测了蓝鳃鱼鱼苗（BF-2）、大鳞鲑胚胎（CHSE-214）、鲤乳头状上皮瘤（EPC）、黑头呆鱼（FHM）和虹鳟鱼性腺（RTG-2）等5种鱼类细胞系中的支原体污染。该方法不适用于监测BF-2、CHSE-214、FHM和RTG-2细胞系，因为它们掺入了尿嘧啶。尿苷和尿嘧啶的差异掺入可能适用于筛选EPC细胞，因为只有该细胞系可以区分实验性感染口腔支原体的培养物与已知不含微生物污染物的培养物。
. Differential incorporation of uridine and uracil was used to assay for mycoplasma contamination in five fish cell lines: bluegill fry (BF-2), chinook salmon embryo (CHSE-214), epithelioma papillosum cyprini (EPC), fathead minnow (FHM) and rainbow trout gonad (RTG-2). The method was not suitable for monitoring BF-2, CHSE-214, FHM, and RTG-2 cell lines because they incorporated uracil. Differential incorporation of uridine and uracil may be applicable for screening EPC cells because only this cell line could distinguish cultures experimentally infected with Mycoplasma orale from cultures known to be free from microbial contaminants.