Exercise and lymphocyte activation following chemotherapy for breast cancer
Exercise and lymphocyte activation following chemotherapy for breast cancer
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DOI：
10.1249/01.mss.0000175857.84936.1a
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发表时间：
2005-11-01
期刊：
影响因子：
--
通讯作者：
Mastro, AM
中科院分区：
文献类型：
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作者：
Hutnick, NA;Williams, NI;Mastro, AM
目的：确定运动训练是否会增加化疗后乳腺癌患者的淋巴细胞活化。激活是通过 CD4(+)CD69(+) T 辅助淋巴细胞的存在、丝裂原诱导的增殖以及丝裂原刺激的淋巴细胞和患者血浆中产生的细胞因子水平来确定的。方法：将参加 6 个月锻炼计划的乳腺癌患者 (N = 28) 与未锻炼的患者 (N = 21) 进行比较。化疗后、3 个月和 6 个月后，患者接受健康评估并抽血。锻炼计划包括阻力训练和有氧运动，每周 3 次，在私人教练的指导下进行 60-75% 的功能能力。采用免疫化学和流式细胞仪检测CD4(+)CD69(+)血淋巴细胞数量。用刀豆球蛋白 A (ConA)、植物血凝素 (PHA) 或商陆有丝分裂原 (PWM) 刺激全血以确定增殖潜力。采用酶联免疫吸附测定（ELISA）测定促细胞分裂原刺激的淋巴细胞培养基中干扰素-γ（IFN-γ）和白细胞介素-6（IL-6）的浓度以及血浆中IL-6、可溶性IL-6受体、可溶性gp130和IFN-γ的浓度。使用 Wilcoxon 符号秩检验对不同时间的组进行分析，并使用 Mann-Whitney U 检验进行组间比较。 结果：运动患者的最大摄氧量和上身力量有所增加。在干预结束时用 ConA、PHA 和 PWM 刺激时，该组还显示出更高百分比的 CD4(+)CD69(+) 细胞和更高水平的氚化胸苷掺入（DNA 合成）。两组中血浆和丝裂原刺激的 IL-6 和 IFN-γ 产生相似。结论：运动可以通过增加乳腺癌患者治疗后的淋巴细胞活化来改善免疫功能。
Purpose: To determine whether exercise training would increase lymphocyte activation in patients with breast cancer following chemotherapy. Activation was determined by the presence of CD4(+)CD69(+) T-helper lymphocytes, mitogen-induced proliferation, and levels of cytokines produced by mitogen- stimulated lymphocytes and in the patients' plasma.Methods: Patients with breast cancer (N = 28) who participated in a 6-month exercise program were compared with patients (N = 21) who did not exercise. Following chemotherapy, and 3 and 6 months later, patients underwent fitness evaluations and had blood drawn. The exercise program consisted of resistance training and aerobic activity at 60-75% functional capacity three times a week with a personal trainer. Immunochemistry and flow cytometry were used to measure the number of CD4(+)CD69(+) blood lymphocytes. Whole blood was stimulated with concanavalin A (ConA), phytohemagglutin (PHA), or pokeweed mitogen (PWM) to determine proliferation potential. Enzyme-linked immunosorbent assays (ELISA) were used to determine the concentration of interferon-gamma (IFN-gamma) and interleukin-6 (IL-6) in the culture medium of mitogen-stimulated lymphocytes as well as the plasma concentrations of IL-6, soluble IL-6 receptor, soluble gp130, and IFN-gamma. Analysis of groups across time was done using the Wilcoxon signed rank test, and comparisons of groups were done using the Mann-Whitney U test.Results: The exercising patients showed increases in maximal oxygen uptake and upper body strength. This group also showed a greater percentage of CD4(+)CD69(+) cells and a greater level of tritiated thymidine incorporation (DNA synthesis) when stimulated with ConA, PHA, and PWM at the end of the intervention. Plasma and mitogen-stimulated IL-6 and IFN-gamma production were similar in both groups.Conclusion: Exercise may improve immune function by increasing lymphocyte activation in patients with breast cancer following treatment.