Circularly permuted AsLOV2 as an optogenetic module for engineering photoswitchable peptides
Circularly permuted AsLOV2 as an optogenetic module for engineering photoswitchable peptides
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DOI：
10.1039/d1cc02643g
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发表时间：
2021-07-22
影响因子：
4.9
通讯作者：
Wang, Wenjing
中科院分区：
文献类型：
--
作者：
Geng, Lequn;Shen, Jiaqi;Wang, Wenjing
我们重新设计了一种常用的光传感蛋白，AsLOV 2，使用环状排列策略，允许光开关控制的C-末端的肽。我们证明了循环排列的AsLOV 2可以单独使用，也可以与原始AsLOV 2一起使用，以增强锁定。总之，循环排列的AsLOV 2可以扩展光遗传学工具的工程能力。
We re-engineered a commonly-used light-sensing protein, AsLOV2, using a circular permutation strategy to allow photoswitchable control of the C-terminus of a peptide. We demonstrate that the circularly permuted AsLOV2 can be used on its own or together with the original AsLOV2 for enhanced caging. In summary, circularly permuted AsLOV2 could expand the engineering capabilities of optogenetic tools.