Impact of high-grain diet feeding on mucosa-associated bacterial community and gene expression of tight junction proteins in the small intestine of goats
Impact of high-grain diet feeding on mucosa-associated bacterial community and gene expression of tight junction proteins in the small intestine of goats
复制标题
高谷物饮食喂养对山羊小肠粘膜相关细菌群落和紧密连接蛋白基因表达的影响。
DOI：
10.1002/mbo3.745
复制
发表时间：
2019-06-01
期刊：
影响因子：
3.4
通讯作者：
Mao, Shengyong
中科院分区：
文献类型：
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作者：
Liu, Junhua;Xue, Chunxu;Mao, Shengyong
本研究旨在探讨高谷物（HG）日粮对山羊小肠微生物发酵、粘膜相关细菌菌群组成和紧密连接蛋白基因表达的影响。在本研究中，我们将10只公山羊随机分配至干草饮食（n = 5）或HG饮食（56.5%谷物; n = 5），然后使用Illumina MiSeq测序检查细菌群落的变化，并使用qRT-PCR检查小肠粘膜中紧密连接蛋白的表达。结果表明，与干草日粮相比，HG日粮降低了回肠肠腔pH（p = 0.005），增加了回肠肠腔中脂多糖含量（p < 0.001），提高了空肠肠腔中总挥发性脂肪酸含量（p = 0.015）和回肠肠腔中总挥发性脂肪酸含量（p = 0.007）。MiSeq测序结果表明，与干草饮食相比，HG饮食增加了空肠粘膜中寡养单胞菌属、莫拉氏菌属、乳杆菌属和普雷沃氏菌属的百分比（FDR = 0.007-0.028），但降低了回肠粘膜中克里斯滕森菌科R7组的丰度（FDR = 0.016）。此外，HG饮食导致空回肠粘膜中claudin-4、occludin和ZO-1的mRNA表达下调（p < 0.05）。总的来说，我们的数据表明，HG饮食诱导的一些粘膜相关细菌的相对丰度的变化，除了在小肠中的紧密连接蛋白的mRNA表达下调。这些研究结果为反刍动物小肠对HG饲喂的适应性反应提供了新的见解。
The objective of this study was to investigate the impact of a high-grain (HG) diet on the microbial fermentation, the composition of the mucosa-associated bacterial microbiota, and the gene expression of tight junction proteins in the small intestine of goats. In the present study, we randomly assigned 10 male goats to either a hay diet (n = 5) or a HG diet (56.5% grain; n = 5) and then examined changes in the bacterial community using Illumina MiSeq sequencing and the expression of tight junction proteins using qRT-PCR in the mucosa of the small intestine. The results showed that HG diet decreased the luminal pH (p = 0.005) and increased the lipopolysaccharide content (p < 0.001) in the digesta of the ileum, and it increased the concentration of total volatile fatty acids in the digesta of the jejunum (p = 0.015) and ileum (p = 0.007) compared with the hay diet. MiSeq sequencing results indicated that the HG diet increased (FDR = 0.007-0.028) the percentage of the genera Stenotrophomonas, Moraxella, Lactobacillus, and Prevotella in jejunal mucosa but decreased (FDR = 0.016) the abundance of Christensenellaceae R7 group in the ileal mucosa compared with the hay diet. Furthermore, the HG diet caused downregulation of the mRNA expression of claudin-4, occludin, and ZO-1 in jejunal and ileal mucosa (p < 0.05). Collectively, our data suggested that the HG diet induced changes in the relative abundance of some mucosa-associated bacteria, in addition to downregulation of the mRNA expression of tight junction proteins in the small intestine. These findings provide new insights into the adaptation response of the small intestine to HG feeding in ruminants.