Preparation of selective micro glucose sensor without permselective membrane by electrochemical deposition of ruthenium and glucose oxidase
Preparation of selective micro glucose sensor without permselective membrane by electrochemical deposition of ruthenium and glucose oxidase
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DOI：
10.1016/j.elecom.2006.12.015
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发表时间：
2007-05-01
影响因子：
5.4
通讯作者：
Kuwabata, Susumu
中科院分区：
文献类型：
--
作者：
Kohma, Takuya;Oyamatsu, Daisuke;Kuwabata, Susumu
研究了一种碳纤维微电极，其表面电化学共沉积了钌和葡萄糖氧化酶（GOx）。微电极上的Ru沉积增加了对H2O2氧化的电流响应，同时由于抗坏血酸、尿酸、对乙酰氨基苯酚、L-半胱氨酸和多巴胺等干扰物质而降低了氧化电流。 Ru 和 GOx 的共沉积进一步抑制了干扰信号，同时保持了对 H2O2 的电流响应。当使用GOx和Ru修饰的微电极进行电流葡萄糖传感时，沉积浴中GOx浓度的增加增大了GOx氧化葡萄糖产生的H2O2的氧化电流。分析物中抗坏血酸的存在不会导致葡萄糖检测出现误差，而对于测量 5 mM 葡萄糖（血液中的正常生理水平），由尿酸引起的误差最多为 +3%。 (c) 2006 Elsevier B.V. 保留所有权利。
A carbon fiber microelectrode, surface of which ruthenium and glucose oxidase (GOx) were electrochemically codeposited, has been investigated. The Ru deposition onto the microelectrode increased current response to H2O2 oxidation, while decreased oxidation currents due to interfering substances, such as ascorbic acid, uric acid, p-acetamidophenol, L-cysteine and dopamine. The codeposition of Ru and GOx gave further suppression of the interfering signals with keeping the current response to H2O2. When amperometric glucose sensing was conducted by using the GOx and Ru modified microelectrode, an increase in GOx concentration in the deposition bath enlarged oxidation current of H2O2 generated from glucose oxidation by GOx. The presence of ascorbic acid in analyte gave no error in detection of glucose and errors caused by uric acid was +3% at the most for measuring 5 mM glucose, which is the normal physiological level in blood. (c) 2006 Elsevier B.V. All rights reserved.