Cost-effective and compact wide-field fluorescent imaging on a cell-phone
Cost-effective and compact wide-field fluorescent imaging on a cell-phone
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DOI：
10.1039/c01c00358a
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发表时间：
2011-01-01
期刊：
影响因子：
6.1
通讯作者：
Ozcan, Aydogan
中科院分区：
文献类型：
--
作者：
Zhu, Hongying;Yaglidere, Oguzhan;Ozcan, Aydogan
我们展示了宽场荧光和暗场成像的手机与紧凑，轻便，具有成本效益的光学元件，机械连接到现有的相机单元的手机。为此，我们使用电池供电的发光二极管（LED）从侧面泵浦感兴趣的样品，使用对接耦合，其中泵浦光在样品比色皿内被引导以均匀地激发样品。然后使用位于手机相机现有透镜正前方的附加透镜对来自样品的荧光发射成像。由于激发是通过垂直于我们的检测路径传播的导波发生的，因此廉价的塑料滤色器足以产生荧光成像所需的暗场背景，而不需要薄膜干涉滤光片。我们通过以2种颜色（即，红色和绿色）在近似于81 mm（2）的大视场（FOV）上，具有近似于20 μ m的原始空间分辨率。与额外的数字处理的捕获的手机图像，通过使用压缩采样理论，我们证明了类似的2倍提高我们的分辨率，实现类似于10 μ m的分辨率，而没有权衡在我们的FOV。此外，我们还演示了暗场成像的非荧光标本使用相同的接口，其中这一次从对象的散射光被检测到，而不使用任何过滤器。成像宽FOV的能力对于探测大样本体积（例如，> 0.1 mL）的E.例如，在一个实施例中，血液、尿液、痰液或水，为此，我们还展示了来自全血样品的标记的白细胞以及水传播的病原性原生动物寄生虫如贾第鞭毛虫包囊的荧光成像。重量仅为28 g（约1盎司），这种连接到手机上的紧凑且具有成本效益的荧光成像平台可能非常有用，特别是对于资源有限的环境，并且可能为宽场成像和为全球健康应用开发的各种芯片实验室检测的定量提供重要工具，例如监测HIV+患者的CD4计数或病毒载量测量。
We demonstrate wide-field fluorescent and darkfield imaging on a cell-phone with compact, lightweight and cost-effective optical components that are mechanically attached to the existing camera unit of the cell-phone. For this purpose, we used battery powered light-emitting diodes (LEDs) to pump the sample of interest from the side using butt-coupling, where the pump light was guided within the sample cuvette to uniformly excite the specimen. The fluorescent emission from the sample was then imaged using an additional lens that was positioned right in front of the existing lens of the cell-phone camera. Because the excitation occurs through guided waves that propagate perpendicular to our detection path, an inexpensive plastic colour filter was sufficient to create the dark-field background required for fluorescent imaging, without the need for a thin-film interference filter. We validate the performance of this platform by imaging various fluorescent micro-objects in 2 colours (i.e., red and green) over a large field-of-view (FOV) of similar to 81 mm(2) with a raw spatial resolution of similar to 20 mu m. With additional digital processing of the captured cell-phone images, through the use of compressive sampling theory, we demonstrate similar to 2 fold improvement in our resolving power, achieving similar to 10 mu m resolution without a trade-off in our FOV. Further, we also demonstrate darkfield imaging of non-fluorescent specimen using the same interface, where this time the scattered light from the objects is detected without the use of any filters. The capability of imaging a wide FOV would be exceedingly important to probe large sample volumes (e.g., > 0.1 mL) of e. g., blood, urine, sputum or water, and for this end we also demonstrate fluorescent imaging of labeled white-blood cells from whole blood samples, as well as water-borne pathogenic protozoan parasites such as Giardia Lamblia cysts. Weighing only similar to 28 g (similar to 1 ounce), this compact and cost-effective fluorescent imaging platform attached to a cell-phone could be quite useful especially for resource-limited settings, and might provide an important tool for wide-field imaging and quantification of various lab-on-a-chip assays developed for global health applications, such as monitoring of HIV+ patients for CD4 counts or viral load measurements.