Free energy change of ATP-hydrolysis: a causal factor of early hypoxic failure of the myocardium?
Free energy change of ATP-hydrolysis: a causal factor of early hypoxic failure of the myocardium?
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ATP 水解自由能变化：心肌早期缺氧衰竭的原因之一？
DOI：
10.1016/0022-2828(82)90205-x
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发表时间：
1982
影响因子：
5
通讯作者：
E. Jüngling
中科院分区：
文献类型：
--
作者：
H. Kammermeier;P. Schmidt;E. Jüngling
5至7分钟期间。用三种类型的灌注介质（A：2m m Ca 2+；B：2m m Ca 2++ 利多卡因；C：6m m Ca 2++ 利多卡因）对离体电起搏大鼠心脏进行缺氧灌注，记录和估计以下参数：心室内峰值压力和规则搏动和配对刺激后搏动的力量，Ca 2+ 推注给药下相同的机械参数，以下代谢物的组织含量：ATP， ADP、磷酸肌酸 (PC)、肌酸 (Cr) 和无机磷酸盐 (P i)。使用CK平衡方程和已发表的组织区室数据计算细胞溶质浓度。根据胞质浓度，计算磷酸化电位 [ATP][ADP][P i] 和 ATP 水解的自由能变化 (dG d ϋ)。收缩表现的过程和组织代谢物含量的变化与文献报道一致。 PC 存储的快速崩溃和相对快速的收缩性能下降伴随着 ATP 的缓慢和中度下降。各分割产物的组织含量互补增加。没有一个单一的代谢参数与收缩性能具有令人满意的相关性。相反，当 ATP 水解的自由能变化（控制为 60.5 kJ/mol）在 50 至 45 kJ/mol 之间时，收缩性能会急剧下降。根据这些结果，至少在缺氧情况下，ATP 水解的低水平自由能变化而不是 ATP 缺乏，可以被认为是收缩衰竭的主要原因之一。
During 5 to 7 min. anoxic perfusion of isolated electrically paced rat hearts with three types of perfusion media (A: 2m m Ca 2+; B: 2m m Ca 2++ lidocaine; C: 6m m Ca 2++ lidocaine), the following parameters were recorded and estimated: peak intraventricular pressure and force of both regular beats and beats following paired stimulation, the same mechanical parameters under Ca 2+ bolus administration, tissue content of the following metabolites: ATP, ADP, phosphocreatine (PC), creatine (Cr), and inorganic phosphate (P i). Cytosolic concentrations were calculated using CK equilibrium equation and published data of tissue compartmentation. From cytosolic concentrations the phosphorylation potential [ATP][ADP][P i] and free energy change of ATP hydrolysis (dG d ξ) were calculated. The course of contractile performance and changes in tissue metabolite content are consistent with those of literature. Fast breakdown of PC stores and relatively fast reduction of contractile performance are accompanied by slow and moderate decrease in ATP. The tissue contents of the respective split products increase complementarily. None of the single metabolic parameters correlates satisfactorily with contractile performance. In contrast, steep reduction of contractile performance occurs when free energy change of ATP hydrolysis (controls 60.5 kJ/mol) is between 50 and 45 kJ/mol. According to these results the low level of free energy change of ATP hydrolysis rather than ATP deficiency, at least under anoxia, can be considered to be one main causal factor of contractile failure.
低浓度的脂肪酸可以抑制钙从肌浆网囊泡中流出。
影响因子：
6.1
作者：
Katz,AM;Messineo,F;Miceli,J;Nash-Adler,PA
通讯作者：
Nash-Adler,PA