Helical repeat and linking number of surface-wrapped DNA.
Helical repeat and linking number of surface-wrapped DNA.
复制标题
表面包裹 DNA 的螺旋重复和连接数。
DOI：
10.1126/science.3388041
复制
发表时间：
1988
期刊：
影响因子：
56.9
通讯作者：
William R. Bauer
中科院分区：
文献类型：
--
作者：
James H. White;N. Cozzarelli;William R. Bauer
当DNA包裹在蛋白质表面时，双链DNA的几何性质会发生系统性的改变。表面缠绕的闭合环状DNA的连接数是两个整数之和：缠绕数，phi，螺旋重复的函数;和表面连接数，SLk，一个新定义的几何常数，解释了表面几何形状对DNA扭曲和扭动的影响。螺旋重复，h，和在绕组数的变化可以推导出单独从表面几何形状和超螺旋密度，西格玛。这种处理将理论上重要的性质twist和writhe与实验上更容易获得的量phi、h、SLk和sigma联系起来。该分析适用于三个生物学上重要的情况下：缠绕的DNA在plectonemic超螺旋，连环DNA，和微型染色体。
The geometric properties of duplex DNA are systematically altered when the DNA is wrapped on a protein surface. The linking number of surface-wrapped closed circular DNA is the sum of two integers: the winding number, phi, a function of the helical repeat; and the surface linking number, SLk, a newly defined geometric constant that accounts for the effects of surface geometry on the twist and writhe of DNA. Changes in the helical repeat, h, and in the winding number can be deduced solely from surface geometry and superhelix density, sigma. This treatment relates the theoretically important properties twist and writhe to the more experimentally accessible quantities phi, h, SLk, and sigma. The analysis is applied to three biologically important cases: interwinding of DNA in a plectonemic superhelix, catenated DNA, and minichromosomes.
影响因子：
5.6
作者：
DREW, HR;TRAVERS, AA
通讯作者：
TRAVERS, AA
扭曲差异在 DNA 结构分析中的应用。
DOI：
10.1073/pnas.85.3.772
发表时间：
1988
影响因子：
11.1
作者：
White,JH;Bauer,WR
通讯作者：
Bauer,WR