Activation of ERK and p38 MAP kinases in human fibroblasts during collagen matrix contraction
Activation of ERK and p38 MAP kinases in human fibroblasts during collagen matrix contraction
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DOI：
10.1006/excr.2000.4866
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发表时间：
2000-05-25
影响因子：
3.7
通讯作者：
Grinnell, F
中科院分区：
文献类型：
--
作者：
Lee, DJ;Rosenfeldt, H;Grinnell, F
进行了研究，以表征在等长张力下成纤维细胞收缩胶原基质期间MAP激酶活化的变化。我们发现，ERK和p38 MAP激酶在收缩过程中被激活，通过免疫印迹和体外激酶测定。ERK激活是双相的，峰值在10分钟和2小时，而p38激活是双相的，在10分钟的一个单一的峰值。ERK的激活，但不是p38，似乎至少部分依赖于G（i）类异源三聚体G蛋白。结果表明，ERK和p38在收缩刺激的c-fos转录激活中协同作用。(C)北京大学出版社.
Studies were carried out to characterize changes in MAP kinase activation during contraction of collagen matrices by fibroblasts under isometric tension. We found that both ERK and p38 MAP kinases were activated during contraction, as determined by immunoblotting and in vitro kinase assays. ERK activation was biphasic, with peaks at 10 min and 2 h; whereas p38 activation was monophasic, with a single peak at 10 min. Activation of ERK, but not p38, appeared to depend at least in part on the G(i) class of heterotrimeric G proteins. The results show that ERK and p38 cooperate in contraction-stimulated activation of c-fos transcription. (C) 2000 Academic Press.