Laser tissue welding analyzed using fluorescence, Stokes shift spectroscopy, and Huang-Rhys parameter.
Laser tissue welding analyzed using fluorescence, Stokes shift spectroscopy, and Huang-Rhys parameter.
复制标题
使用荧光、斯托克斯位移光谱和 Huang-Rhys 参数分析激光组织焊接。
DOI：
10.1002/jbio.201100050
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发表时间：
2012
影响因子：
2.8
通讯作者：
Alfano,RobertR
中科院分区：
文献类型：
--
作者：
Sriramoju,Vidyasagar;Alfano,RobertR
采用1450 nm波长的近红外（NIR）连续波激光辐射，利用组织中天然水的组合振动模式对猪主动脉和皮肤样品进行焊接。在胶原蛋白、弹性蛋白和色氨酸的特定激发和发射波长下，测量了焊接组织和非焊接组织的关键天然分子的荧光光谱。测量关键天然荧光团的荧光强度变化和Stokes位移（Δνss）差异，以区分发色团的Huang‐Rhys参数值(S)。偶联的强度取决于组织内分子环境的局部电子振动和胶原蛋白、弹性蛋白和色氨酸的净电荷周围的极性溶剂水的量。非焊接组织和焊接组织的数值几乎相同且小于3，表明焊接对局部分子环境的影响很小。（©2012 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim）
Near infrared (NIR) continuous wave laser radiation at the 1,450 nm wavelength was used to weld porcine aorta and skin samples via the absorption of combitional vibrational modes of native water in the tissues. The fluorescence spectra were measured from the key native molecules of welded and non‐welded tissues at specific excitation and emission wavelengths from collagen, elastin, and tryptophan. The changes in the fluorescence intensities and differences in Stokes shift (Δνss) of key native fluorophores were measured to differentiate the Huang‐Rhys parameter values (S) of the chromophores. The strength of coupling depends on the local electron‐vibration intra‐tissue molecular environment and the amount of polar solvent water surrounding the net charges on collagen, elastin, and tryptophan. TheSvalues for both non‐welded and welded tissues were almost the same and less than 3, suggesting minimal changes in the local molecular environment as a result of welding. (© 2012 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)