Glucose uptake in rat soleus: effect of acute unloading and subsequent reloading.
Glucose uptake in rat soleus: effect of acute unloading and subsequent reloading.
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大鼠比目鱼肌的葡萄糖摄取：急性卸载和随后重新加载的影响。
DOI：
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发表时间：
1988
影响因子：
3.3
通讯作者：
Marc E. Tischler
中科院分区：
文献类型：
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作者：
Eric J. Henriksen;Marc E. Tischler
采用抛尾和悬吊的方法，研究了急性减重（卸载）对大鼠比目鱼肌2-[1,2- 3h]脱氧d -葡萄糖体外摄取的影响。4 h后，2-脱氧-d -葡萄糖的摄取下降（-19%,P < 0.01）， 20 h后进一步下降。24小时的这种减少与[14C]葡萄糖氧化减慢和[14C]葡萄糖并入糖原有关。与1天后不同，卸载3天后2-脱氧-d -葡萄糖的基础摄取与对照组没有差异。卸载1或3天后重新加载比目鱼肌使2-脱氧-d -葡萄糖的摄取增加到高于控制水平，并分别在6小时和4天内恢复到正常水平。这些卸载和恢复的影响是由比目鱼肌的局部变化引起的，因为来自同一后肢的指长伸肌在摄取2-脱氧-d -葡萄糖或葡萄糖代谢方面没有表现出任何改变。本研究表明，卸载或卸载后的恢复引起的收缩活动的改变可以影响比目鱼肌中葡萄糖运输的调节。
The effect of acutely reduced weight bearing (unloading) on the in vitro uptake of 2-[1,2-3H]deoxy-D-glucose was studied in the soleus muscle by tail casting and suspending rats. After just 4 h, the uptake of 2-deoxy-D-glucose fell (-19%, P less than 0.01) and declined further after an additional 20 h of unloading. This diminution at 24 h was associated with slower oxidation of [14C]glucose and incorporation of [14C]glucose into glycogen. Unlike after 1 day, at 3 days of unloading basal uptake of 2-deoxy-D-glucose did not differ from control. Reloading of the soleus after 1 or 3 days of unloading increased uptake of 2-deoxy-D-glucose above control and returned it to normal within 6 h and 4 days, respectively. These effects of unloading and recovery were caused by local changes in the soleus, because the extensor digitorum longus from the same hindlimbs did not display any alterations in uptake of 2-deoxy-D-glucose or metabolism of glucose. This study demonstrates that alterations in contractile activity, brought about by unloading or recovery from unloading, can influence the regulation of glucose transport in the soleus.