Assessing the Speciation of a Cold Water Species, Japanese Sand Lance Ammodytes personatus, in the Northwestern Pacific by AFLP Markers
Assessing the Speciation of a Cold Water Species, Japanese Sand Lance Ammodytes personatus, in the Northwestern Pacific by AFLP Markers
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通过 AFLP 标记评估西北太平洋冷水物种日本沙矛鱼的物种形成
DOI：
10.3390/ani8120224
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发表时间：
2018-12-01
期刊：
影响因子：
3
通讯作者：
Iida, Koji
中科院分区：
文献类型：
--
作者：
Han, Zhiqiang;Wang, Zhiyong;Iida, Koji
评估高度分化的多个线粒体DNA（mtDNA）谱系的状态并划分进化显著单元（ESU）是预测气候变化对种内遗传变异影响的基础。本研究利用AFLP技术对人工沙蜥的遗传结构进行了研究，并对人工沙蜥的遗传变异进行了比较。在不同的洋流系统中。细胞核分支与先前线粒体谱系之间的不一致性表明，A. personatus实际上是由至少两个遗传上不同的神秘物种组成的。我们的研究结果表明，物种内的多样性应考虑到评估气候变化对物种的影响。这项研究还强调了温暖和寒冷的洋流造成的自然物理环境在揭示温度与物种分布之间的相关性方面的价值。在未来的研究中，在这两个群体的温度的确切范围进行评估，必须有足够数量的样本，以预测全球气候变化对这两个group.Abstract的影响，使用分子生物学技术在生物多样性研究越来越多的结果在识别多个不同的线粒体DNA（mtDNA）谱系低于形态种的水平。然而，多个不同谱系的重叠分布提出了一个问题，即这些谱系中的一些是否实际上是神秘物种。评估这些不同谱系的地位，划定进化显著单位（ESU），并确定占主导地位的进化和生态驱动因素是成功的野生动物保护和管理战略的关键组成部分。应用AFLP分子标记技术研究了冷水性鱼类--日本沙蜥（Ammodytes personatus）在暖、冷海流中的地理分布特征。从黑潮、亲潮、对马流和黄海等12个种群共采集211个个体进行分析。贝叶斯分配概率检验和邻居加入（NJ）分析将这些人口分为两个遗传和地理上不同的分支（北方和南方分支），其特征在于不同的海面温度。细胞核分支与先前线粒体谱系之间的不一致性表明，A. personatus实际上是由至少两个遗传上不同的神秘物种组成的。更新世冰川隔离后，二次接触，当地的热适应，隔离距离可以解释观察到的地理格局的两个神秘的物种和遗传结构内的分支。
Simple Summary Assessing the status of multiple highly divergent mitochondrial DNA (mtDNA) lineages and delimiting the evolutionarily significant units (ESUs) are the foundation of forecasting the influence of climate change on intraspecific genetic variation. In this study, we employed AFLP to investigate the genetic structure of Ammodytes personatus and compared the genetic variation of A. personatus in different ocean current systems. The incongruence between nuclear clades and previous mitochondrial lineages suggested that A. personatus is indeed composed of at least two genetically divergent cryptic species. Our results demonstrate that intra-species diversity should be taken into account to assess the influence of climate change on species. This study also highlights the value of the natural physical setting created by warm and cold ocean currents in eliciting a correlation between temperature and species distribution. In future studies, the exact range of temperature in both groups must be assessed with a sufficient number of samples to predict the influences of global climate change on both groups.Abstract The use of molecular techniques in biodiversity research increasingly results in the recognition of multiple divergent mitochondrial DNA (mtDNA) lineages below the morphospecies level. However, the overlapping distribution of multiple divergent lineages raises the question of whether some of these lineages are in fact cryptic species. Assessing the status of these divergent lineages, delimiting evolutionarily significant units (ESUs), and identifying the dominant evolutionary and ecological drivers are critical components of successful wildlife conservation and management strategies. Amplified fragment length polymorphism (AFLP) markers were applied to characterize the phylogeography pattern of a cold water species, the Japanese sand lance Ammodytes personatus, in warm and cold ocean currents. A total of 211 individuals sampled from 12 populations through the species' range, including samples from Kuroshio Current, Oyashio Current, Tsushima Current, and Yellow Sea, were analyzed. The Bayesian assignment probability test and Neighbor joining (NJ) analysis divided these populations into two genetically and geographically distinct clades (northern and southern clades) characterized by different sea surface temperatures. The incongruence between nuclear clades and previous mitochondrial lineages suggested that A. personatus is indeed composed of at least two genetically divergent cryptic species. Pleistocene glaciation isolation after secondary contact, local thermal adaptation, and isolation by distance may explain the observed geographic pattern of two cryptic species and genetic structure within clades.