Attomole Catechins Determination by Capillary Liquid Chromatography with Electrochemical Detection
Attomole Catechins Determination by Capillary Liquid Chromatography with Electrochemical Detection
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DOI：
10.2116/analsci.23.157
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发表时间：
2007-02
影响因子：
1.6
通讯作者：
Akira Kotani;Kouji Takahashi;H. Hakamata;S. Kojima;F. Kusu
中科院分区：
文献类型：
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作者：
Akira Kotani;Kouji Takahashi;H. Hakamata;S. Kojima;F. Kusu
用毛细管液相色谱-电化学检测系统(CLC-ECD)测定了儿茶素的摩尔质量分数，并将该系统应用于人血浆中儿茶素的测定。儿茶素(C)、表儿茶素(EC)、没食子儿茶素(GC)、表儿茶素没食子酸酯(EGC)、没食子儿茶素没食子酸酯(CG)、表儿茶素没食子酸酯(CG)、没食子儿茶素没食子酸酯(GCG)和表没食子儿茶素没食子酸酯(EGCG)在10分钟内用毛细管柱(0.2 mm i.d.)分离。流动相为磷酸(85%)-甲醇-水(0.5：27.5：72.5，v/v/v)，检测电压为+0.85V vs。Ag/AgCl.研究发现，峰高与注射的儿茶素的量呈线性关系，范围从200amol到500fmo1(r>；0.998)。儿茶素的检出限分别为：EGC 61amol，EC 75amol，GC 54amol，C 61amol，GCG 67amol，EGCG 75amol，心电75amol，CG amol(S/N=3)。由于本方法灵敏度高，可方便地对血浆样品进行前处理，因此可测定10μL进食绿茶后血浆中儿茶素及其结合物的浓度时程。
Attomole quantities of catechins were determined by a capillary liquid chromatography system with electrochemical detection (CLC-ECD) and the system is applied to the determination of catechins in human plasma. The eight catechins: catechin (C), epicatechin (EC), gallocatechin (GC), epigallocatechin (EGC), catechin gallate (Cg), epicatechin gallate (ECg), gallocatechin gallate (GCg), and epigallocatechin gallate (EGCg), were separated within 10 min using a capillary column (0.2 mm i.d.) and a mobile phase of phosphoric acid (85%)-methanol-water (0.5:27.5:72.5, v/v/v), and were detected at +0.85 V vs . Ag/AgCl. Peak heights were found to be linearly related to the amount of catechins injected, from 200 amol to 500 fmol ( r > 0.998). The detection limits of the catechins were 61 amol for EGC, 75 amol for EC, 54 amol for GC, 61 amol for C, 67 amol for GCg, 75 amol for EGCg, 75 amol for ECg and 89 amol for Cg ( S / N = 3). Because the present method is highly sensitive and allows facile pretreatment for plasma sample, the time courses of concentrations of catechins (GCg, EC, EGCg, ECg, and Cg) and their conjugates in human plasma obtained from a 10 μl plasma sample after ingestion of green tea could be determined.