Expression failure of the Notch signaling system is associated with the pathogenesis of testicular germ cell tumor
Expression failure of the Notch signaling system is associated with the pathogenesis of testicular germ cell tumor
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DOI：
10.1159/000079140
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发表时间：
2004-01-01
期刊：
影响因子：
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通讯作者：
Kihara, K
中科院分区：
文献类型：
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作者：
Hayashi, T;Yamada, T;Kihara, K
目的：检测睾丸生殖细胞肿瘤中细胞类型的关键因子Notch 1及其配体Jagge2的表达，探讨其与睾丸生殖细胞肿瘤发病机制的关系。方法：对139例睾丸生殖细胞肿瘤标本进行Northern印迹分析和Notch1、Jagge2免疫组织化学染色。结果：Notch 1和Jagge2转录本在非癌睾丸组织中均有表达，在精子细胞中呈阳性表达。精原细胞瘤中Jagge2和Notch 1的转录和蛋白表达均为阴性，胚胎癌和绒毛膜癌中Jagge2和Notch 1均为阴性，畸胎瘤中Jagge2和Notch 1均为阳性。结论：我们的结果首次提供了Notch信号系统的活性与生殖细胞肿瘤的不同组织学亚型相关的可能性，这可能与肿瘤细胞对化疗和/或放射的反应有关。版权所有(C)2004 S.Karger AG，巴塞尔。
Objectives: The expression of Notch 1 and its ligand Jagged 2, critical factors in cell type specification, in testicular germ cell tumors was examined in order to evaluate its possible relationship with their pathogenesis. Methods: Northern blot analysis and immunohistochemical staining for Notch 1 and Jagged 2 were done in 139 samples of testicular germ cell tumors. Results: Notch 1 and Jagged 2 transcripts were expressed in noncancerous testicular tissues and Notch 1 and Jagged 2 proteins were positive in the spermatids. However, the expressions of transcript and protein were negative for both Jagged 2 and Notch 1 in seminomas, they were negative for Jagged 2 but were positive for Notch 1 in embryonal carcinomas and choriocarcinomas, and they were positive for Jagged 2 and Notch 1 in teratomas. Conclusions: Our results offer, for the first time, the possibility that the activity of the Notch signaling system, one of the cell proliferation and differentiation pathways, correlates with the different histological subtypes of germ cell tumor, which may be responsible for characteristics of cancer cells such as responses to chemotherapy and/or irradiation. Copyright (C) 2004 S. Karger AG, Basel.