High-Throughput Screening of Drugs Against the Growth of Cryptosporidium parvum In Vitro by qRT-PCR.
High-Throughput Screening of Drugs Against the Growth of Cryptosporidium parvum In Vitro by qRT-PCR.
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通过 qRT-PCR 体外高通量筛选抑制小隐孢子虫生长的药物。
DOI：
10.1007/978-1-4939-9748-0_18
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发表时间：
2020
期刊：
影响因子：
--
通讯作者：
Zhu,Guan
中科院分区：
文献类型：
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作者：
Zhang,Haili;Zhu,Guan
有效的寄生虫载量定量方法是体外抗隐孢子虫药物疗效评价的关键。然而，通过劳动密集型的传统方法，在体外高通量筛选抗微小隐孢子虫药物是不现实的。在这里，我们描述了一种简化的定量RT-PCR方法，适用于化合物的HTS和评估药物对FC生长的疗效。体外细小病毒。
An effective method to quantify the parasite loads is the key to the evaluation of anti-cryptosporidial drug efficacy in vitro. However, high-throughput screening (HTS) of drugs againstCryptosporidium parvumin vitro was impractical by the labor-intensive traditional assays. Here we describe a simplified quantitative RT-PCR assay suitable for HTS of compounds and for evaluating drug efficacy against the growth ofC. parvumin vitro.