RRS1 gene expression involved in the progression of papillary thyroid carcinoma.
RRS1 gene expression involved in the progression of papillary thyroid carcinoma.
复制标题
RRS1基因表达参与甲状腺乳头状癌的进展
DOI：
10.1186/s12935-018-0519-x
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发表时间：
2018
影响因子：
5.8
通讯作者：
Ni X
中科院分区：
文献类型：
--
作者：
Chen F;Jin Y;Feng L;Zhang J;Tai J;Shi J;Yu Y;Lu J;Wang S;Li X;Chu P;Han S;Cheng S;Guo Y;Ni X
背景甲状腺乳头状癌（Papillary Thyroid Carcinoma，PTC）是最常见的内分泌系统恶性肿瘤之一，其发病机制、进展及预后尚不清楚。尽管越来越多的证据强调核糖体生物合成调节同源物（RRS1）作为一种核糖体生物合成蛋白在酵母和植物，鲜为人知的是人类RRS1 function.MethodsProliferation，细胞周期和凋亡的PTC细胞进行了评估后，敲低RRS1表达通过MTT，集落形成试验，流式细胞仪。然后，进行转录组谱分析以探索PTC细胞中RRS1沉默后的通路变化。接受者工作特征曲线和约登指数进行了24甲状腺癌样本，以评估其潜在的临床诊断value.ResultsFirst，我们发现，沉默RRS1显着降低细胞增殖，抑制细胞周期，并促进PTC细胞系凋亡。RRS1基因敲低可上调与细胞凋亡和代谢相关的基因表达，下调与细胞增殖和血管发育相关的基因表达。值得注意的是，本研究证实了RRS1的诊断价值为甲状腺癌在儿童和adults.ConclusionsIn结论，这些数据提供了一个全面的观点，一个新的功能，人类RRS1通过促进细胞增殖，并可能是一个潜在的指标乳头状甲状腺癌。
BackgroundPapillary thyroid carcinoma (PTC) is one of the most frequent malignancies of the endocrine system, whose mechanisms of pathogenesis, progression and prognosis are still far from being clearly elucidated. Despite an increasing body of evidences highlights ribosome biogenesis regulator homolog (RRS1) as a ribosome biogenesis protein in yeast and plants, little is known about human RRS1 function.MethodsProliferation, cell cycle and apoptosis of PTC cells were assessed following the knockdown of RRS1 expression though MTT, colony formation assay, and flow cytometry. Then, transcriptome profiling was conducted to explore pathway changes after RRS1 silencing in PTC cells. Receiver operating characteristic curve and Youden’s index were performed in twenty-four thyroid carcinoma samples to assess their potential clinical diagnostic value.ResultsFirstly, we found that silencing RRS1 significantly reduced cell proliferation, inhibited cell cycle, and promoted apoptosis in PTC cell line. The result also showed that knock-down of RRS1 could up-regulate genes involving apoptosis and metabolism, while, down-regulate genes relative to cell proliferation and blood vessel development. Notably, the present study confirmed the diagnostic value of RRS1 for thyroid carcinoma in both children and adults.ConclusionsIn conclusion, these data afford a comprehensive view of a novel function of human RRS1 by promoting cell proliferation and could be a potential indicator for papillary thyroid carcinoma.