Conversion of bulk seashells to biocompatible hydroxyapatite for bone implants
Conversion of bulk seashells to biocompatible hydroxyapatite for bone implants
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DOI：
10.1016/j.actbio.2007.06.003
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发表时间：
2007-11-01
影响因子：
9.7
通讯作者：
Kim, Choll W.
中科院分区：
文献类型：
--
作者：
Vecchio, Kenneth S.;Zhang, Xing;Kim, Choll W.
Strombus gigas（海螺）壳和Tridacna gigas（巨蛤）壳具有致密的定制结构，赋予这些壳优异的机械性能。以海螺和文蛤为原料，采用水热法在不同温度和不同转化时间下制备羟基磷灰石。在类似于200摄氏度的相对低温下，在大多数样品中由这些壳产生了致密的HAP结构。发现部分转化的海螺壳样品的平均断裂应力类似于137-218 MPa，原始和转化的蛤壳样品的平均断裂应力类似于70-150 MPa，这接近于致密人骨的机械强度。这表明转换后的髋臼杯样本可用作承重情况下的植入物。在大鼠股骨缺损中进行6周的转换壳体样品的体内测试。6周时的显微断层扫描图像显示植入物没有移动，未处理的对照缺损仍然是空的，没有自发融合的证据。组织学研究表明，有新形成的骨生长到种植体及其周围。植入物周围没有纤维化组织环的证据，也表明植入物没有松动。相比之下，未处理的对照保持空的，有一些证据表明缺损孔周围有纤维化环。这些结果表明，转化壳植入物具有良好的生物相容性和生物活性。(C)2007 Acta Materialia Inc.由爱思唯尔有限公司出版。保留所有权利。
Strombus gigas (conch) shells and Tridacna gigas (Giant clam) shells have dense, tailored structures that impart excellent mechanical properties to these shells. In this investigation, conch and clam seashells were converted to hydroxyapatite (HAP) by a hydrothermal method at different temperatures and for different conversion durations. Dense HAP structures were created from these shells throughout the majority of the samples at the relative low temperature of similar to 200 degrees C. The average fracture stress was found to be similar to 137-218 MPa for partially converted conch shell samples and similar to 70-150 MPa for original and converted clamshell samples, which is close to the mechanical strength of compact human bone. This indicates that the converted shell samples can be used as implants in load-bearing cases. In vivo tests of converted shell samples were performed in rat femoral defects for 6 weeks. The microtomography images at 6 weeks show that the implants did not move, and untreated control defects remain empty with no evidence of a spontaneous fusion. Histological study reveals that there is newly formed bone growing up to and around the implants. There is no evidence of a fibrosis tissue ring around the implants, also indicating that there is no loosening of the implants. In contrast,, the untreated controls remain empty with some evidence of a fibrosis ring around the defect hole. These results indicate good biocompatibility and bioactivity of the converted shell implants. (C) 2007 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.