STIMULATORY EFFECT OF OPIOID-PEPTIDES AND NALOXONE ON RAT SPINAL-CORD CELLS IN PRIMARY DISSOCIATED CULTURE
STIMULATORY EFFECT OF OPIOID-PEPTIDES AND NALOXONE ON RAT SPINAL-CORD CELLS IN PRIMARY DISSOCIATED CULTURE
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DOI：
10.1016/0736-5748(94)90035-3
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发表时间：
1994-08-01
影响因子：
1.8
通讯作者：
KALENTCHUK, V
中科院分区：
文献类型：
--
作者：
KOZLOVA, M;KALENTCHUK, V
研究了阿片肽leu-脑啡肽、脑啡肽的合成类似物dalargin和阿片受体阻滞剂纳洛酮对大鼠脊髓解离培养细胞的形态学影响。细胞低密度播种(3.10(5)；在胶原底物上进行了6.10(5)个细胞/ml的实验，以证明阿片肽增加了培养细胞的数量和神经突的生长，并导致聚集体形成的初始过程的激活。在以聚l -赖氨酸为底物的高密度电镀（5.10(6)个细胞/ml）时，证明了聚集体形成过程的激活，阿片肽和纳洛酮都导致聚集体大小的增加。对这组实验结果的统计处理表明，与对照组相比，亮脑啡肽、dalargin和纳洛酮的聚集体大小分别增加了2.2倍、2.2倍至2.6倍、2.4倍，即脊髓细胞对阿片肽和阿片受体阻滞剂纳洛酮的反应是单向的。与对照组相比，阿片肽和纳洛酮的总效应导致聚集体的大小增加3.6倍。本研究获得的数据允许假设阿片肽和纳洛酮在通过不同类型的受体激活脊髓细胞的同时，表现出因子的特性，从而增加培养脊髓细胞的存活率和粘附性。
Opioid peptides leu-enkephalin, a synthetic analog of enkephalin dalargin and an opiate receptor blocker naloxone were studied for their morphological effect on the cells of dissociated cultures of rat spinal cord.Low density seeding of cells (3.10(5); 6.10(5) cells/ml) on collagen substrate was performed to document that opioid peptides increase the number of cultured cells and neurite outgrowth and lead to the activation of the initiated processes of aggregate formation. Upon higher density of plating (5.10(6) cells/ml) with poly-L-lysine as a substrate, activation of the aggregate formation process was demonstrated, both opioid peptides and naloxone leading to an increase in the size of aggregates. Statistical treatment of the results obtained in this set of experiments documented that leu-enkephalin, dalargin and naloxone increased 2.2-, 2.2-2.6-, 2.4-fold, respectively, the size of aggregates compared to the control, i.e. the reaction of spinal cord cells to opioid peptides and opiate receptor blocker naloxon was unidirected. The total effect of opioid peptides and naloxon resulted in a 3.6-fold increase in the size of the aggregates compared to the control. The data obtained in this study allow the assumption that opioid peptides and naloxone, while activating spinal cord cells via receptors of a different type, manifest the properties of factors thus increasing survival and adhesion of spinal cord cells in culture.