Clustered organization and region-specific identities of estrogen-producing neurons in the forebrain of Zebra Finches (Taeniopygia guttata).
Clustered organization and region-specific identities of estrogen-producing neurons in the forebrain of Zebra Finches (Taeniopygia guttata).
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DOI：
10.1002/cne.24292
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发表时间：
2017-12-01
期刊：
影响因子：
--
通讯作者：
Remage-Healey L
中科院分区：
文献类型：
--
作者：
Ikeda MZ;Krentzel AA;Oliver TJ;Scarpa GB;Remage-Healey L
据报道，雌激素信号在脊椎动物大脑的许多区域具有快速的神经调节作用。几个物种中芳香酶（雌激素合酶）细胞分布的区域差异表明，神经雌激素信号传导机制在大脑区域之间和内部存在差异。对神经雌激素信号传导的更全面的理解取决于表达芳香酶的神经元的细胞特性。小清蛋白和钙结合蛋白等钙结合蛋白是中间神经元亚型的分子标记，并与人类颞叶皮层中的芳香酶共表达。像斑胸草雀这样的鸣禽已经成为了解雌激素等类固醇的大脑合成及其对神经生物学和行为的影响的重要模型。在这里，我们研究了斑胸草雀听觉和感觉运动前脑中表达芳香酶的神经元的细胞结构和细胞特性的区域差异。芳香酶与小清蛋白在尾内侧巢皮质 (NCM) 和 HVC 架（专有名称）中共表达，但不在尾外侧巢皮质 (NCL) 或海马中共表达。相比之下，钙结合蛋白在所研究的任何区域中均未与芳香酶共表达。值得注意的是，在密集的体细胞簇中发现了表达芳香酶的神经元，这表明局部神经雌激素从簇状神经元中协调释放。与女性相比，男性 NCM 中的芳香酶簇也更加丰富且紧密。总体而言，这项研究为网络水平的神经雌激素调节提供了新的见解，并通过将芳香酶神经元的子集识别为假定的抑制性中间神经元，扩展了人类皮质先前的发现。
A fast, neuromodulatory role for estrogen signaling has been reported in many regions of the vertebrate brain. Regional differences in the cellular distribution of aromatase (estrogen synthase) in several species suggest that mechanisms for neuroestrogen signaling differ between and within brain regions. A more comprehensive understanding of neuroestrogen signaling depends on characterizing the cellular identities of neurons that express aromatase. Calcium-binding proteins such as parvalbumin and calbindin are molecular markers for interneuron subtypes, and are co-expressed with aromatase in human temporal cortex. Songbirds like the zebra finch have become important models to understand the brain synthesis of steroids like estrogens and the implications for neurobiology and behavior. Here, we investigated the regional differences in cytoarchitecture and cellular identities of aromatase-expressing neurons in the auditory and sensorimotor forebrain of zebra finches. Aromatase was co-expressed with parvalbumin in the caudomedial nidopallium (NCM) and HVC shelf (proper name) but not in the caudolateral nidopallium (NCL) or hippocampus. By contrast, calbindin was not co-expressed with aromatase in any region investigated. Notably, aromatase-expressing neurons were found in dense somato-somatic clusters, suggesting a coordinated release of local neuroestrogens from clustered neurons. Aromatase clusters were also more abundant and tightly packed in the NCM of males as compared to females. Overall, this study provides new insights into neuroestrogen regulation at the network level, and extend previous findings from human cortex by identifying a subset of aromatase neurons as putative inhibitory interneurons.
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影响因子：
3.3
作者：
Fukuda, T;Kosaka, T
通讯作者：
Kosaka, T
DOI：
10.1016/s0960-0760(97)00080-0
发表时间：
1997-09-01
影响因子：
4.1
作者：
Baillien, M;Balthazart, J
通讯作者：
Balthazart, J
影响因子：
2.7
作者：
Comins, Jordan A.;Gentner, Timothy Q.
通讯作者：
Gentner, Timothy Q.
影响因子：
2.9
作者：
Gahr, M;GarciaSegura, LM
通讯作者：
GarciaSegura, LM
影响因子：
4.8
作者：
Balthazart, J;Baillien, M;Ball, GF
通讯作者：
Ball, GF