A reliable isotope dilution method for simultaneous determination of fumonisins B1, B2 and B3 in traditional Chinese medicines by ultra-high-performance liquid chromatography-tandem mass spectrometry
A reliable isotope dilution method for simultaneous determination of fumonisins B1, B2 and B3 in traditional Chinese medicines by ultra-high-performance liquid chromatography-tandem mass spectrometry
复制标题
DOI：
10.1002/jssc.201000423
复制
发表时间：
2010-09-01
影响因子：
3.1
通讯作者：
Wu, Yongjiang
中科院分区：
文献类型：
--
作者：
Han, Zheng;Ren, Yiping;Wu, Yongjiang
建立了同位素稀释超高效液相色谱-串联质谱法同时测定中药中伏马菌素B1、伏马菌素B2和伏马菌素B3的方法，并对提取、净化、超高效液相色谱分离和串联质谱条件进行了优化。样品经50%乙腈水溶液提取，MultiSep 211 Fum柱净化。采用线性梯度移动的流动相（由含0.2%甲酸的水和乙腈/甲醇（50：50 v/v）组成）和Acquity UPLC HSS T3色谱柱（100 mm x 2.1 mm，1.8 μ m），以获得用于正ESI+分析的分析物的最佳分离度。方法的线性关系（R-2 >= 0.9991）、灵敏度（定量限，0.08-0.16 ng/mL）、回收率（88.2-113.3%）和精密度（RSD
A reliable isotope dilution method for simultaneous determination of fumonisin B1, fumonisin B2 and fumonisin B3 in traditional Chinese medicines by ultra-high-performance LC-MS/MS was developed, and a special focus was placed on the optimization of extraction, cleanup, ultra-high-performance LC separation and MS/MS conditions. Homogenized samples were extracted by 50% acetonitrile aqueous solution and purified with MultiSep 211 Fum columns. A linear gradient mobile phase, consisting of water containing 0.2% formic acid and acetonitrile/methanol (50:50 v/v) and an Acquity UPLC HSS T3 column (100 mm x 2.1 mm, 1.8 mu m) were employed to obtain the best resolution of the analytes for the positive ESI+ analysis. The established method was validated by determining the linearity (R-2 >= 0.9991), sensitivity (LOQ, 0.08-0.16 ng/mL), recovery (88.2-113.3%) and precision (RSD