PsANT, the adenine nucleotide translocase of Puccinia striiformis, promotes cell death and fungal growth.
PsANT, the adenine nucleotide translocase of Puccinia striiformis, promotes cell death and fungal growth.
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PsANT 是条锈菌的腺嘌呤核苷酸转位酶，可促进细胞死亡和真菌生长
DOI：
10.1038/srep11241
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发表时间：
2015-06-10
影响因子：
4.6
通讯作者：
Kang Z
中科院分区：
文献类型：
--
作者：
Tang C;Wei J;Han Q;Liu R;Duan X;Fu Y;Huang X;Wang X;Kang Z
腺嘌呤核苷酸转位酶（ANT）是酵母和哺乳动物线粒体的组成性组分，参与ADP/ATP交换和腺苷酸介导的细胞凋亡。然而，ANT在病原真菌中的功能知之甚少。在本研究中，我们确定了一个ANT基因的小麦条锈菌f。（Pst），命名为PsANT。PsANT蛋白含有三个典型的保守的线粒体载体蛋白（mito-carr）结构域，与其它真菌的同源蛋白有70%以上的同源性，表明ANT在真菌中是保守的。免疫细胞化学定位证实了PsANT在正常Pst菌丝细胞或塌陷细胞中的线粒体定位。PsANT的过表达表明PsANT促进烟草、小麦和裂殖酵母细胞中的细胞死亡。进一步的研究表明，三个mito-carr结构域都是诱导细胞死亡所必需的。qRT-PCR分析揭示了在感染期间植物内诱导的PsANT表达。利用宿主诱导的基因沉默系统（HIGS）敲低PsANT，在感染早期减弱了强毒Pst的生长和发育，但不足以改变其致病性。这些结果为PsANT在真菌细胞死亡和生长中的功能提供了新的见解，并可能有助于寻找和设计新的疾病控制策略。
Adenine nucleotide translocase (ANT) is a constitutive mitochondrial component that is involved in ADP/ATP exchange and mitochondrion-mediated apoptosis in yeast and mammals. However, little is known about the function of ANT in pathogenic fungi. In this study, we identified an ANT gene of Puccinia striiformis f. sp. tritici (Pst), designated PsANT. The PsANT protein contains three typical conserved mitochondrion-carrier-protein (mito-carr) domains and shares more than 70% identity with its orthologs from other fungi, suggesting that ANT is conserved in fungi. Immuno-cytochemical localization confirmed the mitochondrial localization of PsANT in normal Pst hyphal cells or collapsed cells. Over-expression of PsANT indicated that PsANT promotes cell death in tobacco, wheat and fission yeast cells. Further study showed that the three mito-carr domains are all needed to induce cell death. qRT-PCR analyses revealed an in-planta induced expression of PsANT during infection. Knockdown of PsANT using a host-induced gene silencing system (HIGS) attenuated the growth and development of virulent Pst at the early infection stage but not enough to alter its pathogenicity. These results provide new insight into the function of PsANT in fungal cell death and growth and might be useful in the search for and design of novel disease control strategies.
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腺嘌呤核苷酸易位酶-1是渗透性过渡孔的成分，可以主要诱导凋亡。
影响因子：
7.8
作者：
Bauer, M K;Schubert, A;Rocks, O;Grimm, S
通讯作者：
Grimm, S
影响因子：
4.4
作者：
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通讯作者：
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影响因子：
3.9
作者：
LEQUOC, K;LEQUOC, D
通讯作者：
LEQUOC, D
影响因子：
20.1
作者：
GRIENDLING, KK;MINIERI, CA;ALEXANDER, RW
通讯作者：
ALEXANDER, RW
DOI：
10.1073/pnas.90.17.7951
发表时间：
1993-09-01
影响因子：
11.1
作者：
LOO, DT;COPANI, A;COTMAN, CW
通讯作者：
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