Alterations of molecular properties of lipoxygenase induced by dense phase carbon dioxide
Alterations of molecular properties of lipoxygenase induced by dense phase carbon dioxide
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密相二氧化碳诱导脂氧合酶分子特性的改变
DOI：
10.1016/j.ifset.2008.06.007
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发表时间：
2009-01-01
影响因子：
6.6
通讯作者：
Wu, Jihong
中科院分区：
文献类型：
--
作者：
Liao, Xiaojun;Zhang, Yan;Wu, Jihong
用透射电子显微镜、十二烷基硫酸钠-聚丙烯酰胺凝胶和天然PAGE、远紫外圆二色谱和荧光光谱分析了致密相二氧化碳(DPCD)诱导脂氧合酶(LOX)分子性质的变化。经DPCD处理后，LOX的粒径增大，说明LOX分子聚集。在55℃的DPCD作用30min后，由于聚集作用，在Native-PAGE上观察到LOX条带的缺失，但在SDS-PAGE上LOX的电泳性没有改变。DPCD处理的LOX的CID和荧光光谱发生了明显的变化，其α-螺旋相对含量急剧下降到10%以下，其固有相对荧光强度(RFI)随着压力的增加而线性下降。工业相关性：DPCD作为一种新型的非热技术，可以保持待加工食品的质量。然而，DPCD使食品酶失活的机理直到今天才被阐明。本研究有助于了解该技术的作用机理，推动该技术在食品工业中的应用。(C)2008爱思唯尔有限公司。保留所有权利。
Alterations of molecular properties of lipoxygenase (LOX) induced by dense phase carbon dioxide (DPCD) were analyzed using transmission electronic microscope (TEM), SDS-PAGE and native-PAGE, far UV-circular dichroism (CD) and fluorescence spectroscopy. The aggregation of LOX molecules was suggested by an increase in the particle size of LOX after DPCD treatment. The absence of LOX band was observed after exposure to DPCD at 55 degrees C for 30 min on the native-PAGE due to the aggregation, but the electrophoretic behavior of LOX was not altered on the SDS-PAGE. The CID and fluorescence spectra of DPCD-treated LOX were noticeably changed, its alpha-helix relative content decreased sharply to less than 10%, and its intrinsic relative fluorescence intensity (RFI) decreased linearly with increasing pressures.Industrial relevance: As a novel non-thermal technology, DPCD can retain the quality of foods to be processed. However, the inactivation mechanism of food enzymes by DPCD is not elucidated until today. This investigation is beneficial to understand the mechanism and to push the application of this technology in food industry. (C) 2008 Elsevier Ltd. All rights reserved.