Soya protein β-conglycinin ameliorates fatty liver and obesity in diet-induced obese mice through the down-regulation of PPARγ
Soya protein β-conglycinin ameliorates fatty liver and obesity in diet-induced obese mice through the down-regulation of PPARγ
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DOI：
10.1017/s0007114518000739
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发表时间：
2018-06-14
影响因子：
3.6
通讯作者：
Yamazaki, Tomomi
中科院分区：
文献类型：
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作者：
Li, Dongyang;Ikaga, Reina;Yamazaki, Tomomi
高脂肪饮食会导致肥胖和非酒精性脂肪肝（NAFLD）。肥胖和NAFLD的改善是一个重要的问题。β-伴大豆球蛋白是一种大豆蛋白，已知可预防糖尿病、肥胖和NAFLD。因此，我们的目的是研究β-伴大豆球蛋白对改善高脂饮食诱导的肥胖小鼠的肥胖和NAFLD的作用，并阐明肝脏和白色脂肪组织（WAT）中这些作用的机制。将DIO雄性ddY小鼠分为6组：HF、中脂（MF）和低脂（LF）组，分别饲喂酪蛋白，HF、MF和LF组均用β-伴大豆球蛋白替代酪蛋白。5周后，补充β-伴大豆球蛋白的组导致较低的体重、皮下WAT的相对重量和肝脏TAG含量（P = 0.001）。此外，β-伴大豆球蛋白抑制HF饮食组中Ppar γ 2、固醇调节元件结合蛋白-1c和靶基因的肝脏表达。在HF饮食组中，与喂食酪蛋白的小鼠相比，喂食β-伴大豆球蛋白的小鼠的附睾和皮下WAT中炎症相关基因的表达显著降低。此外，Ppar γ 1和Ppar γ 2 mRNA的表达在HF饮食组的皮下WAT中被抑制，但在附睾WAT中不被抑制。在喂食β-伴大豆球蛋白的小鼠的血清中，胰岛素和瘦素的浓度较低。总之，β-伴大豆球蛋白有效地改善DIO小鼠的肥胖和NAFLD，并且它似乎是用于改善NAFLD和肥胖的有希望的膳食蛋白质。
Diets high in fat can result in obesity and non-alcoholic fatty liver disease (NAFLD). The improvement of obesity and NAFLD is an important issue. beta-Conglycinin, one of the soya proteins, is known to prevent hyperlipidaemia, obesity and NAFLD. Therefore, we aimed to investigate the effects of beta-conglycinin on the improvement of obesity and NAFLD in high-fat (HF) diet-induced obese (DIO) mice and clarify the mechanism underlying these effects in liver and white adipose tissue (WAT). DIO male ddY mice were divided into six groups: HF, mediumfat (MF) and low-fat (LF) groups fed casein, and HF, MF and LF groups in all of which the casein was replaced by beta-conglycinin. A period of 5 weeks later, the beta-conglycinin-supplemented group resulted in lower body weight, relative weight of subcutaneous WAT, and hepatic TAG content (P = 0.001). Furthermore, beta-conglycinin suppressed the hepatic expression of Ppar gamma 2 in the HF dietary group, sterol regulatory element-binding protein-1c and the target genes. The expressions of inflammation-related genes were significantly low in the epididymal and subcutaneous WAT from the mice fed beta-conglycinin compared with those fed casein in the HF dietary group. Moreover, the expressions of Ppar gamma 1 and Ppar gamma 2 mRNA were suppressed in subcutaneous WAT in the HF dietary group but not in epididymal WAT. The concentrations of insulin and leptin were low in the serum of the mice fed beta-conglycinin. In conclusion, beta-conglycinin effectively improved obesity and NAFLD in DIO mice, and it appears to be a promising dietary protein for the amelioration of NAFLD and obesity.