MORPHOLOGIES OF RABBIT RETINAL GANGLION-CELLS WITH CONCENTRIC RECEPTIVE-FIELDS
MORPHOLOGIES OF RABBIT RETINAL GANGLION-CELLS WITH CONCENTRIC RECEPTIVE-FIELDS
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DOI：
10.1002/cne.902800107
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发表时间：
1989-02-01
影响因子：
2.5
通讯作者：
OYSTER, CW
中科院分区：
文献类型：
--
作者：
AMTHOR, FR;TAKAHASHI, ES;OYSTER, CW
兔视网膜神经节细胞与同心感受野细胞内记录和染色的隔离灌流眼杯准备与特定的生理反应特性的树突状形态。同心神经节细胞，如传统定义的，是那些具有开或关中心的拮抗性周围，但缺乏复杂的响应特性，如方向或取向选择性。同心圆细胞被分为不同的组，其上或偏离中心的反应信号，兴奋性场中心的大小，线性的空间总和，轻快与缓慢和瞬态与持续响应光强度的阶跃变化的细胞外记录。然后将细胞刺穿，在细胞内记录期间确认身份，并用辣根过氧化物酶离子电渗注射用于组织学分析。回收23个同心神经节细胞并进行形态计量学分析。它们的生理反应特性被发现与细胞树突状分支模式的一些潜在的二维和三维属性有关。所有20个活跃的同心细胞和三个呆滞细胞中的两个的树突被发现分支狭窄的近端或远端的一半的内丛状层，对应于它们是否在中心或偏离中心，分别。发现其中一个迟缓的同心细胞具有更复杂的、部分双层的分支。生理学鉴定轻快持续线性，轻快瞬态非线性，轻快瞬态线性，至少有两类迟缓同心神经节细胞染色。这些生理类别中的每一个似乎都表现出独特的和可识别的树突状分支模式。
Rabbit retinal ganglion cells with concentric receptive fields were intracellularly recorded and stained in the isolated superfused eyecup preparation to relate specific physiological response properties to dendritic morphology. Concentric ganglion cells, as traditionally defined, were those that had On or Off centers with antagonistic surrounds but lacked complex response properties such as direction or orientation selectivity. Concentric cells were classified into different groups by extracellular recordings of their On- or Off-center response sign, excitatory field center size, linearity of spatial summation, and brisk vs. sluggish and transient vs. sustained responses to step changes in light intensity. The cells were then impaled, confirmed in identity during intracellular recording, and iontophoretically injected with horseradish peroxidase for histological analysis. Twenty-three concentric ganglion cells were recovered and morphometrically analyzed. Their physiological response properties were found to be related to a number of underlying two- and three-dimensional attributes of the cell''s dendritic branching patterns. The dendrites of all 20 brisk concentric cells and two of the three sluggish cells were found to ramify narrowly in either the proximal or distal half of the inner plexiform layer, corresponding to whether they are On center or Off center, respectively. One of the sluggish concentric cells was found to have a more complex, partially bistratified ramification. Physiologically identified brisk-sustained-linear, brisk transient-nonlinear, brisk-transient-linear, and at least two classes of sluggish concentric ganglion cells were stained. Each of these physiological classes appears to exhibit a distinct and identifiable dendritic branching pattern.