Tissue Microarray: A rapidly evolving diagnostic and research tool.
Tissue Microarray: A rapidly evolving diagnostic and research tool.
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DOI：
10.4103/0256-4947.51806
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发表时间：
2009-03
影响因子：
1.6
通讯作者：
Jawhar NM
中科院分区：
文献类型：
--
作者：
Jawhar NM
组织微阵列是病理学领域的一项新技术。微阵列包含来自数百个不同病例的许多小的代表性组织样本，这些样本组装在单个组织学载玻片上，因此允许同时对多个标本进行高通量分析。组织微阵列是通过从不同的石蜡供体块中提取圆柱形组织核心并将其重新包埋到定义的阵列坐标处的单个受体（微阵列）块中而产生的石蜡块。使用这种技术，多达1000个或更多的组织样本可以排列成一个单一的石蜡块。它可以允许在同一标准化条件下在单个载玻片上同时分析DNA，mRNA和蛋白质水平的分子靶标，并且还可以最大限度地保存和使用有限且不可替代的存档组织样本。这种多功能的技术，其中数据分析是自动化的，有利于回顾性和前瞻性的人体组织研究。它是一种实用而有效的组织高通量分子分析工具，有助于识别人类癌症中新的诊断和预后标志物和靶标，并在基础研究，预后肿瘤学和药物发现中具有一系列潜在应用。本文综述了组织芯片构建和切片的技术方面，优点，应用和局限性。
Tissue microarray is a recent innovation in the field of pathology. A microarray contains many small representative tissue samples from hundreds of different cases assembled on a single histologic slide, and therefore allows high throughput analysis of multiple specimens at the same time. Tissue microarrays are paraffin blocks produced by extracting cylindrical tissue cores from different paraffin donor blocks and re-embedding these into a single recipient (microarray) block at defined array coordinates. Using this technique, up to 1000 or more tissue samples can be arrayed into a single paraffin block. It can permit simultaneous analysis of molecular targets at the DNA, mRNA, and protein levels under identical, standardized conditions on a single glass slide, and also provide maximal preservation and use of limited and irreplaceable archival tissue samples. This versatile technique, in which data analysis is automated facilitates retrospective and prospective human tissue studies. It is a practical and effective tool for high-throughput molecular analysis of tissues that is helping to identify new diagnostic and prognostic markers and targets in human cancers, and has a range of potential applications in basic research, prognostic oncology and drug discovery. This article summarizes the technical aspects of tissue microarray construction and sectioning, advantages, application, and limitations.