Thymopoietin-like substance in human skin.
Thymopoietin-like substance in human skin.
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人体皮肤中的胸腺生成素样物质。
DOI：
10.1111/1523-1747.ep12517686
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发表时间：
1983
期刊：
影响因子：
--
通讯作者：
Edelson,RL
中科院分区：
文献类型：
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作者：
Chu,AC;Patterson,JA;Goldstein,G;Berger,CL;Takezaki,S;Edelson,RL
异源抗胸腺生成素(抗-TP)抗体被用来确定表皮中是否存在类似TP的分子，因为这些因素被认为在已知的T细胞-表皮细胞相互作用中发挥了作用。用间接免疫荧光染色对新鲜分离的人表皮细胞进行细胞离心涂片检查，发现8-14%的细胞与抗TP抗体发生胞浆反应。同样，在组织培养中保存2-8周的人表皮细胞中，2-5%的细胞显示抗TP抗体的胞浆染色。用抗TP抗体和与朗格汉斯细胞(OKT6)特异反应的单抗进行双标记免疫荧光研究表明，含有该类TP物质的细胞不是朗格汉斯细胞。对正常人皮肤4-μm冰冻切片的原位研究表明，具有TP样物质的细胞群是表皮角质形成细胞的基底层。
A heterologous antithymopoietin (anti-TP) antibody was used to determine whether a TP-like molecule is present in the epidermis, since such factors have been postulated to play a part in known T cell-epidermal cell interaction. Examination of cytocentrifuge smears of freshly separated human epidermal cells stained by indirect immunofluorescence revealed that 8–14% of these cells possessed cytoplasmic reactivity with the anti-TP antibody. Similarly, 2–5% of human epidermal cells, maintained in tissue culture for 2-8 weeks, showed cytoplasmic staining with the anti-TP antibody. Double-labeling immunofluorescence studies, with the anti-TP antibody and a monoclonal antibody specifically reactive with Langerhans cells (OKT6), demonstrated that cells possessing this TP-like substance were not Langerhans cells. In situ studies of 4-μm frozen sections of normal human skin indicated that the cell population which possesses the TP-like substance is the basal layer of keratincoytes in the epidermis.