Lymph Node Subcapsular Sinus Microenvironment-On-A-Chip Modeling Shear Flow Relevant to Lymphatic Metastasis and Immune Cell Homing.
Lymph Node Subcapsular Sinus Microenvironment-On-A-Chip Modeling Shear Flow Relevant to Lymphatic Metastasis and Immune Cell Homing.
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DOI：
10.1016/j.isci.2020.101751
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发表时间：
2020-11-20
期刊：
影响因子：
5.8
通讯作者：
Thomas SN
中科院分区：
文献类型：
--
作者：
Birmingham KG;O'Melia MJ;Bordy S;Reyes Aguilar D;El-Reyas B;Lesinski G;Thomas SN
设计了一种淋巴结窦-芯片粘附微流体平台，其重现了淋巴结被膜下窦的流体动力学微环境。该装置用于询问淋巴结重塑对与淋巴转移相关的流体流动中的细胞粘附的影响。壁剪切应力水平分析估计和建模后，静止和患病/发炎的淋巴结实验重演使用基于流动的微流体灌注系统，以评估人体转移性癌细胞粘附的生理流场的影响。结果表明，这两个改变的流体流动概况和介绍的粘合剂配体，这是预测表现在淋巴结包膜下窦作为炎症诱导的重塑的结果，和淋巴细胞的存在下，淋巴传播的单核细胞可能协同有助于细胞粘附的动态范围内流动相关的淋巴结癌细胞和单核细胞免疫细胞在淋巴转移过程中的淋巴结侵袭。淋巴结被膜下窦芯片被设计用于研究淋巴转移改变的流体流动和粘附配体调节被膜下窦中的细胞粘附单核细胞以淋巴结流动依赖的方式增加癌细胞粘附粘附淋巴结重塑的生物物理效应调节淋巴转移机制组织工程;癌症;生物工程
A lymph node sinus-on-a-chip adhesion microfluidic platform that recapitulates the hydrodynamic microenvironment of the lymph node subcapsular sinus was engineered. This device was used to interrogate the effects of lymph node remodeling on cellular adhesion in fluid flow relevant to lymphatic metastasis. Wall shear stress levels analytically estimated and modeled after quiescent and diseased/inflamed lymph nodes were experimentally recapitulated using a flow-based microfluidic perfusion system to assess the effects of physiological flow fields on human metastatic cancer cell adhesion. Results suggest that both altered fluid flow profiles and presentation of adhesive ligands, which are predicted to manifest within the lymph node subcapsular sinus as a result of inflammation-induced remodeling, and the presence of lymph-borne monocytic cells may synergistically contribute to the dynamic extent of cell adhesion in flow relevant to lymph node invasion by cancer and monocytic immune cells during lymphatic metastasis. A lymph node subcapsular sinus-on-a-chip was engineered to study lymphatic metastasis Altered fluid flows and adhesive ligands regulate cell adhesion in subcapsular sinus Monocytes increase cancer cell adhesion adhesion in a lymph node flow-dependent manner Biophysical effects of lymph node remodeling regulates lymphatic metastasis mechanisms Tissue Engineering; Cancer; Bioengineering
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影响因子：
9.3
作者：
Edwards, Erin Elizabeth;Birmingham, Katherine Gayle;Thomas, Susan Napier
通讯作者：
Thomas, Susan Napier
DOI：
10.1196/annals.1413.020
发表时间：
2008-01-01
期刊：
LYMPHATIC CONTINUUM REVISITED
影响因子：
--
作者：
Achen, Marc G.;Stacker, Steven A.
通讯作者：
Stacker, Steven A.
影响因子：
7.3
作者：
Berendam, Stella J.;Koeppel, Alexander F.;Engelhard, Victor H.
通讯作者：
Engelhard, Victor H.
预防转移的药物开发。
影响因子：
--
作者：
Fontebasso Y;Dubinett SM
通讯作者：
Dubinett SM
影响因子：
4.4
作者：
Faveeuw, C;Di Mauro, ME;Ager, A
通讯作者：
Ager, A