TYPE-I AND TYPE-II MECHANISMS IN THE PHOTOSENSITIZED LYSIS OF PHOSPHATIDYLCHOLINE LIPOSOMES BY HEMATOPORPHYRIN
TYPE-I AND TYPE-II MECHANISMS IN THE PHOTOSENSITIZED LYSIS OF PHOSPHATIDYLCHOLINE LIPOSOMES BY HEMATOPORPHYRIN
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DOI：
10.1111/j.1751-1097.1982.tb04358.x
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发表时间：
1982-01-01
影响因子：
3.3
通讯作者：
GROSSWEINER, JB
中科院分区：
文献类型：
--
作者：
GROSSWEINER, LI;PATEL, AS;GROSSWEINER, JB
磷脂酰胆碱（PC）脂质体的裂解通过掺入脂质体（0.09- 1.5%，wt/wt）或外部缓冲液（1-15 μ M）中的血卟啉（HP）对可见光（> 500 nm）敏感。裂解机制从低HP浓度下由单线态氧（1 O2）介导的II型途径变为高HP浓度下的缺氧I型途径。HP在水溶液和有机溶剂中的光谱测量表明，HP不聚集（单体和/或二聚体）的II型敏化和聚集的I型条件。用粗血卟啉衍生物（HPD）的初步实验显示出类似的浓度效应的裂解效率，其中PC脂质体纳入0.1%（重量/重量）HPD强烈敏化氧，而敏化氧是微不足道的3.1% HPD。HP和粗制Hpd的结果表明，生物膜中的溶解性损伤不一定需要氧合。
The lysis of phosphatidylcholine (PC) liposomes was sensitized to visible light (> 500 nm) by hematoporphyrin (HP) incorporated in the liposomes (0.09-1.5%, wt/wt) or in the external buffer (1-15 .mu.M). The lytic mechanism changed from the Type II pathway mediated by singlet oxygen (1O2) at low HP concentrations to the anoxic, type I pathway at high HP concentrations. Spectral measurements of HP in aqueous and organic solvents indicate that the HP was not aggregated (monomers and/or dimers) for type II sensitization and aggregated for Type I conditions. Preliminary experiments with crude hematoporphyrin derivative (Hpd) showed similar concentration effects on lytic efficiency, where PC liposomes incorporating 0.1% (wt/wt) Hpd were strongly sensitized by oxygen, whereas sensitization by oxygen was insignificant with 3.1% Hpd. The results with HP and crude Hpd indicate that lytic damage in a biomembrane does not necessarily require oxygenation.