Human platelets can discriminate between various bacterial LPS isoforms via TLR4 signaling and differential cytokine secretion
Human platelets can discriminate between various bacterial LPS isoforms via TLR4 signaling and differential cytokine secretion
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DOI：
10.1016/j.clim.2012.09.004
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发表时间：
2012-12-01
影响因子：
8.6
通讯作者：
Cognasse, Fabrice
中科院分区：
文献类型：
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作者：
Berthet, Julien;Damien, Pauline;Cognasse, Fabrice
目前，血小板被认为是先天免疫和炎症细胞，在脓毒症中发挥着复杂的作用。我们研究了不同类型的脂多糖对血小板释放可溶性信号/有效分子是否有不同的影响。我们使用来自健康志愿者的富含血小板的血浆和来自两种具有不同 LPS 结构的革兰氏阴性菌菌株的 LPS。我们将 LPS 刺激的血小板上清液与报告细胞结合，并测量 PBMC 细胞因子分泌谱。用大肠杆菌 0111 和明尼苏达沙门氏菌 LPS 刺激血小板后，血小板 LPS::TLR4 相互作用激活途径，触发大量分子的产生。不同的血小板上清液导致 PBMC 分泌 IL-6、TNF α 和 IL-8 的差异。我们的数据表明，血小板有能力通过单一类型的病原体识别受体差异地感知外部信号，并针对表现出不同类型“危险”信号的病原体适当调整先天免疫反应。 (C) 2012 Elsevier Inc. 保留所有权利。
Platelets are currently acknowledged as cells of innate immunity and inflammation and play a complex role in sepsis. We examined whether different types of LPS have different effects on the release of soluble signaling/effective molecules from platelets. We used platelet-rich plasma from healthy volunteers and LPS from two strains of gram-negative bacteria with disparate LPS structures. We combined LPS-stimulated platelet supernatants with reporter cells and measured the PBMC cytokine secretion profiles. Upon stimulation of platelets with both Escherichia coli 0111 and Salmonella minnesota LPS, the platelet LPS::TLR4 interaction activated pathways to trigger the production of a large number of molecules. The different platelet supernatants caused differential PBMC secretion of IL-6, TNF alpha, and IL-8. Our data demonstrate that platelets have the capacity to sense external signals differentially through a single type of pathogen recognition receptor and adjust the innate immune response appropriately for pathogens exhibiting different types of 'danger' signals. (C) 2012 Elsevier Inc. All rights reserved.