Protective effect of soybeans as protein source in the diet against cadmium-aorta redox and morphological alteration.
Protective effect of soybeans as protein source in the diet against cadmium-aorta redox and morphological alteration.
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DOI：
10.1016/j.taap.2013.07.016
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发表时间：
2013-11
影响因子：
3.8
通讯作者：
M. F. Pérez Díaz;M. Acosta;F. Mohamed;M. Ferramola;L. Oliveros;M. Giménez
中科院分区：
文献类型：
--
作者：
M. F. Pérez Díaz;M. Acosta;F. Mohamed;M. Ferramola;L. Oliveros;M. Giménez
我们研究了镉暴露对胸主动脉氧化还原状态和形态学的影响，以及大豆在diet.Male Wistar大鼠中的保护作用，分为6组：3个饲料中含有酪蛋白和3个含有大豆，作为蛋白质源。在每个蛋白质组中，一个是自来水（对照）和其他两个自来水分别含有15和100 ppm的镉2+，两个月，在酪蛋白饲料喂养的大鼠，15 ppm的镉诱导硫代巴比妥酸反应物质（TBARS），过氧化氢酶（CAT）和谷胱甘肽过氧化物酶（GPx）的活性增加，100 ppm Cd ~（2+）暴露组大鼠主动脉组织中SOD活性、CAT、GPX、SOD、Nrf 2和金属硫蛋白Ⅱ mRNA表达及CAT、GPx和NOX-2蛋白水平均高于对照组。主动脉内皮细胞和细胞质的变化。然而，与对照组相比，大豆饮食，15和100 ppm的Cd 2+并没有改变TBARS水平; CAT，GPX和Nrf 2 mRNA的表达; CAT，GPx和NOX-2蛋白;和主动脉形态。大豆饮食减弱氧化还原变化，并以剂量依赖性的方式，对镉诱导的主动脉形态学改变提供保护。
We investigated the effects of cadmium exposition on thoracic aorta redox status and morphology, and the putative protective effect of soybeans in the diet.Male Wistar rats were separated into 6 groups: 3 fed with a diet containing casein and 3 containing soybeans, as protein source. Within each protein group, one was given tap water (control) and the other two tap water containing 15 and 100 ppm of Cd2 +, respectively, for two months.In rats fed with casein diet, 15 ppm of Cd induced an increase of thiobarbituric acid-reactive substances (TBARS), and of the catalase (CAT) and glutathione peroxidase (GPx) activities, which were even higher with 100 ppm of Cd2 +, in aorta.Also, 100 ppm Cd2 +exposure increased superoxide dismutase (CuZnSOD) activity; CAT, GPX, SOD, Nrf2 and metallothioneine II mRNA expressions and CAT, GPx and NOX-2 protein levels, compared with control. Aorta endothelial and cytoplasmic alterations were observed.However, with the soybeans diet, 15 and 100 ppm of Cd2 +did not modify TBARS levels; CAT, GPX and Nrf2 mRNA expressions; CAT, GPx and NOX-2 protein; and the aorta morphology, compared with control.The soybean diet attenuates the redox changes and protects against morphological alterations induced, in a dose-dependent way, by Cd in aorta.