Enhancement of meniscal repair in the avascular zone using mesenchymal stem cells in a porcine model
Enhancement of meniscal repair in the avascular zone using mesenchymal stem cells in a porcine model
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DOI：
10.1302/0301-620x.92b1.22629
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发表时间：
2010-01-01
影响因子：
--
通讯作者：
Hui, J. H. P.
中科院分区：
文献类型：
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作者：
Dutton, A. Q.;Choong, P. F.;Hui, J. H. P.
我们假设，与单独使用传统缝合技术治疗的半月板撕裂相比，用间充质干细胞（MSC）结合传统缝合技术治疗的半月板撕裂会显示出更好的愈合效果。在一项对照实验室研究中，28 只成年猪（56 个膝盖）在创建代表撕裂的放射状切口后接受了半月板手术。第 1 组（n = 9）有放射状半月板撕裂，但未进行治疗。第 2 组 (n = 19) 的切口采用缝合线和纤维蛋白胶进行修复，第 3 组（实验组，n = 28）的切口采用 MSC、缝合和纤维蛋白胶进行治疗。 第八周时，第 1 组的宏观检查显示任何标本均未愈合。在第 2 组中，12 个样本未发现愈合，7 个样本未完全愈合。实验组3有21个标本完全愈合，5个不完全愈合，2个未愈合。在实验组和每个对照组之间，这种差异是显着的（p < 0.001）。组织学和肉眼观察结果表明，MSC 显着改善了无血管区半月板撕裂的修复，但愈合后的半月板的机械性能仍然降低。
We hypothesised that meniscal tears treated with mesenchymal stem cells (MSCs) together with a conventional suturing technique would show improved healing compared with those treated by a conventional suturing technique alone. In a controlled laboratory study 28 adult pigs (56 knees) underwent meniscal procedures after the creation of a radial incision to represent a tear. Group 1 (n = 9) had a radial meniscal tear which was left untreated. In group 2 (n = 19) the incision was repaired with sutures and fibrin glue and in group 3, the experimental group (n = 28), treatment was by MSCs, suturing and fibrin glue.At eight weeks, macroscopic examination of group 1 showed no healing in any specimens. In group 2 no healing was found in 12 specimens and incomplete healing in seven. The experimental group 3 had 21 specimens with complete healing, five with incomplete healing and two with no healing. Between the experimental group and each of the control groups this difference was significant (p < 0.001).The histological and macroscopic findings showed that the repair of meniscal tears in the avascular zone was significantly improved with MSCs, but that the mechanical properties of the healed menisci remained reduced.