The stability of Staphylococcal bacteriophages with commonly used prosthetic joint infection lavage solutions.
The stability of Staphylococcal bacteriophages with commonly used prosthetic joint infection lavage solutions.
复制标题
葡萄球菌噬菌体与常用假体关节感染灌洗液的稳定性。
DOI：
10.1002/jor.25731
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发表时间：
2024
期刊：
影响因子：
--
通讯作者：
Urish,KenL
中科院分区：
文献类型：
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作者：
Doub,JamesB;Fogel,Jessa;Urish,KenL
本研究的目的是评估在人工关节感染 (PJI) 手术治疗中，四种葡萄球菌噬菌体暴露于不同浓度的常用灌洗液时的生存能力。本实验使用四种有尾葡萄球菌噬菌体和六种浓度为 100%、1% 和 0.01% 的不同灌洗液（氯己定 4%、过氧化氢 3%、乙酸 3%、聚维酮碘 10%、次氯酸钠 0.5% 和 Vashe 溶液）。此外，还评估了将噬菌体暴露于这些灌洗液中5分钟和24小时的时间影响。结果表明，所有灌洗溶液（100% 和 1%）的四种噬菌体滴度在暴露 5 分钟和 24 小时时均出现统计显着下降。然而，与生理盐水对照相比，在浓度为 0.01% 的灌洗液中，只有乙酸导致噬菌体滴度出现统计学显着下降。我们的研究结果表明，有尾的葡萄球菌噬菌体在高浓度的最常用灌洗液中并不能保持稳定。然而，在非常稀的浓度下，噬菌体仍然保持活力。这具有重要的临床影响，因为它表明，当使用噬菌体治疗 PJI 时，在引入治疗性噬菌体之前彻底清洗所有灌洗液至关重要，尤其是在使用乙酸时。
The aim of this study was to assess the viability of four Staphylococcal bacteriophages when exposed to different concentrations of commonly used lavage solutions in the surgical treatment of prosthetic joint infections (PJI). Four tailed Staphylococcal bacteriophages and six different lavage solutions (chlorhexidine 4%, hydrogen peroxide 3%, acetic acid 3%, povidone iodine 10%, sodium hypochlorite 0.5%, and Vashe solution) at 100%, 1%, and 0.01% concentrations were used in this experiment. In addition, the temporal impact of exposing bacteriophages to these lavage solutions was also evaluated at 5‐min exposures and 24‐h exposures. The results show that the titers of the four bacteriophages were statistically significantly decreased for all lavage solutions (100% and 1%) at 5‐min exposures and 24‐h exposures. However, with 0.01% concentrations of the lavage solutions, only acetic acid caused a statistically significant decrease in bacteriophage titers compared to normal saline control. Our findings suggest that tailed Staphylococcal bacteriophages do not remain stable in high concentrations of the most commonly used lavage solutions. However, at very dilute concentrations the bacteriophages do remain viable. This has important clinical ramifications in that it shows when using bacteriophage therapy for PJI it is critical to thoroughly wash out any lavage solutions before the introduction of therapeutic bacteriophages especially when acetic acid is used.