The roles of RelA/(p)ppGpp in glucose-starvation induced adaptive response in the zoonotic Streptococcus suis.
The roles of RelA/(p)ppGpp in glucose-starvation induced adaptive response in the zoonotic Streptococcus suis.
复制标题
RelA/(p)ppGpp 在人畜共患猪链球菌葡萄糖饥饿诱导的适应性反应中的作用
DOI：
10.1038/srep27169
复制
发表时间：
2016-06-03
影响因子：
4.6
通讯作者：
Zhou R
中科院分区：
文献类型：
--
作者：
Zhang T;Zhu J;Wei S;Luo Q;Li L;Li S;Tucker A;Shao H;Zhou R
（p）ppGpp信号分子在细菌适应营养饥饿的严格反应（SR）中起着重要作用，然而碳水化合物饥饿诱导的适应性反应以及SR在这种反应中的作用还没有得到很好的表征，特别是在革兰氏阳性菌中。在此，在猪链球菌（Streptococcus suis）（一种重要的新兴的人畜共患革兰氏阳性细菌）中鉴定了两种（p）ppGpp合成酶RelA和RelQ，而只有RelA在葡萄糖饥饿下起作用。研究RelA/（p）ppGpp在葡萄糖饥饿反应中的作用。suis，比较了突变株ΔrelA [a（p）ppGpp 0葡萄糖饥饿株]与其亲本株SC-19 [（p）ppGpp+]的生长曲线和转录谱。结果表明，SC-19和ΔrelA在葡萄糖饥饿时的适应性反应上存在很大差异，说明RelA/（p）ppGpp在葡萄糖饥饿适应中起重要作用。除了包括抑制生长和相关大分子合成的经典SR之外，扩展的适应性反应还包括抑制糖酵解和碳分解代谢物抑制（CCR）介导的碳水化合物依赖性代谢开关。总的来说，葡萄糖饥饿诱导的适应性反应的表型和基因型特征在S。suis的研究为更好地理解SR的机制做出了贡献。
The (p)ppGpp signal molecules play a central role in the stringent response (SR) to adapt to nutrient starvation in bacteria, yet the carbohydrate starvation induced adaptive response and the roles of SR in this response is not well characterized, especially in Gram-positives. Here, two (p)ppGpp synthetases RelA and RelQ are identified in Streptococcus suis, an important emerging zoonotic Gram-positive bacterium, while only RelA is functional under glucose starvation. To characterize the roles of RelA/(p)ppGpp in glucose starvation response in S. suis, the growth curves and transcriptional profiles were compared between the mutant strain ΔrelA [a (p)ppGpp0 strain under glucose starvation] and its parental strain SC-19 [(p)ppGpp+]. The results showed great difference between SC-19 and ΔrelA on adaptive responses when suffering glucose starvation and demonstrated that RelA/(p)ppGpp plays important roles in adaptation to glucose starvation. Besides the classic SR including inhibition of growth and related macromolecular synthesis, the extended adaptive response also includes inhibited glycolysis and carbon catabolite repression (CCR)-mediated carbohydrate-dependent metabolic switches. Collectively, the pheno- and genotypic characterization of the glucose starvation induced adaptive response in S. suis makes a great contribution to understanding better the mechanism of SR.