Postnatal changes in the distribution and density of neuronal nuclei and doublecortin antigens in domestic chicks (Gallus domesticus)
Postnatal changes in the distribution and density of neuronal nuclei and doublecortin antigens in domestic chicks (Gallus domesticus)
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DOI：
10.1002/cne.22696
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发表时间：
2012-01-01
影响因子：
2.5
通讯作者：
Csillag, Andras
中科院分区：
文献类型：
--
作者：
Mezey, Szilvia;Krivokuca, Dragan;Csillag, Andras
为了更好地了解窝后雏鸡的神经发生率和新神经元的分布，我们描述并比较了窝后2天、8天和14周雏鸡全脑中神经元核蛋白（NeuN，又称Fox-3）和双皮质素抗原的表达情况。在注射溴脱氧尿嘧啶后24小时和7天（分别为产后2天和8天），比较了雏鸡前脑室和室旁区溴脱氧尿嘧啶标记细胞、NeuN-标记细胞和双皮质素标记细胞的密度。NeuN标记的神经元分布与nissl染色组织相似，除了一些神经元不表达NeuN的区域：小脑浦肯野细胞和嗅球二尖瓣细胞。2日龄雏鸡的腹侧被盖区也有微弱的标记。双皮质素在所有时间点的分布相似，双皮质素标记的分布分布在所有前脑区域以及小脑颗粒细胞层。然而，在任何中脑或脑干区域均未检测到双皮质素标记。我们的数据表明，在孵化后的家鸡中，端脑仍然形成了大量的新神经元，而端脑下区域（小脑除外）在孵化前完成了神经元的扩张。小鸡中大多数新形成的细胞在孵化后离开室旁区，但有一群神经元留在室旁区附近，并在7天内原地成熟。增殖细胞常沿前脑层向仍在发育的脑区横向迁移。J. Comp.神经科。, 2012年。(c) 2011 Wiley期刊公司
To understand better the rate of neurogenesis and the distribution of new neurons in posthatch domestic chicks, we describe and compare the expression of the neuronal nuclei protein (NeuN, a.k.a. Fox-3) and doublecortin antigens in the whole brain of chicks 2 days, 8 days, and 14 weeks posthatch. In the forebrain ventricular and paraventricular zones, the density of bromodeoxyuridine-, NeuN-, and doublecortin-labeled cells was compared between chicks 24 hours and 7 days after an injection of bromodeoxyuridine (2 and 8 days posthatch, respectively). The distribution of NeuN-labeled neurons was similar to Nissl-stained tissue, with the exception of some areas where neurons did not express NeuN: cerebellar Purkinje cells and olfactory bulb mitral cells. The ventral tegmental area of 2-day-old chicks was also faintly labeled. The distribution of doublecortin was similar at all timepoints, with doublecortin-labeled profiles located throughout all forebrain areas as well as in the cerebellar granule cell layer. However, doublecortin labeling was not detectable in any midbrain or brainstem areas. Our data indicate that a significant number of new neurons is still formed in the telencephalon of posthatch domestic chicks, whereas subtelencephalic areas (except for the cerebellum) finish their neuronal expansion before hatching. Most newly formed cells in chicks leave the paraventricular zone after hatching, but a pool of neurons stays in the vicinity of the ventricular zone and matures in situ within 7 days. Proliferating cells often migrate laterally along forebrain laminae into still-developing brain areas. J. Comp. Neurol., 2012. (c) 2011 Wiley Periodicals, Inc.