Protein stapling via azide-alkyne ligation
Protein stapling via azide-alkyne ligation
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DOI：
10.1039/c4cc06528j
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发表时间：
2014-01-01
影响因子：
4.9
通讯作者：
Link, A. James
中科院分区：
文献类型：
--
作者：
Abdeljabbar, Diya M.;Piscotta, Frank J.;Link, A. James
在这里，我们展示了一种称为蛋白质钉接的方法，用于将共价约束引入重组蛋白。采用叠氮-炔咔嗒反应作为钉接化学，提高了模型亮氨酸拉链蛋白的热稳定性。此外，钉接小的球状蛋白G的核心可改善其与靶标免疫球蛋白G的结合。
Here we demonstrate a methodology, termed protein stapling, for the introduction of covalent constraints into recombinant proteins. Using the azide-alkyne click reaction as the stapling chemistry, we have improved the thermostability of a model leucine zipper protein. Additionally, stapling the core of the small, globular protein G resulted in improved binding to its target, immunoglobulin G.