Size and structure of the hydrophobic low molecular weight surfactant-associated polypeptide.
Size and structure of the hydrophobic low molecular weight surfactant-associated polypeptide.
复制标题
疏水性低分子量表面活性剂缔合多肽的大小和结构。
DOI：
10.1021/bi00410a002
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发表时间：
1988
期刊：
影响因子：
2.9
通讯作者：
H. Jörnvall
中科院分区：
文献类型：
--
作者：
J. Johansson;T. Curstedt;B. Robertson;H. Jörnvall
肺表面活性物质中含量最多的低分子量蛋白具有不同寻常的特性。它的初级结构现在已经通过蛋白质水平的分析确定了。高疏水性多肽对蛋白水解酶的裂解具有抗性，但在浓HCl或液氨中与钠的有限裂解可以产生片段。肽的酸水解需要特殊的条件来释放所有残基。n端是异质的，在其最长的形式，初级结构由35个残基组成。这一分析表明，主要的天然疏水表面活性剂多肽的大小比以前提出的要小得多。体外脉动泡法和体内新生家兔实验证实了多肽与磷脂复合的生物学效应。该分子在大约三分之二的位置上具有支链氨基酸残基，并且缺少九种残基。中间的三分之一完全由疏水残基组成，这部分的碎片即使在有机溶剂中也很少可溶。疏水区前面是一个更亲水的n端段。因此，这种分子有两个截然不同的部分，就像一种清洁剂，这可以解释它在肺表面活性剂系统中的重要作用。
The most abundant low molecular weight protein of pulmonary surfactant has unusual properties. Its primary structure has now been determined by analysis at the protein level. The highly hydrophobic polypeptide is resistant to cleavage with proteolytic enzymes, but it was possible to generate fragments by limited cleavage with concentrated HCl or with sodium in liquid ammonia. Acid hydrolysis of the peptide required exceptional conditions for release of all residues. The N-terminus is heterogeneous, and in its longest form the primary structure consists of 35 residues. This analysis establishes that the size of the major native hydrophobic surfactant polypeptide is considerably smaller than previously proposed. Biological effects of the polypeptide recombined with phospholipids are confirmed in vitro by using a pulsating bubble system and in vivo by using premature newborn rabbits. The molecule has branched-chain amino acid residues at about two-thirds of all positions and lacks nine types of residue. The middle third is composed entirely of hydrophobic residues, and fragments from this part are sparingly soluble even in organic solvents. The hydrophobic region is preceded by a more hydrophilic, N-terminal segment. Thus, the molecule has two contrasting parts, like a detergent, which may explain its essential role in the pulmonary surfactant system.
DOI：
10.1073/pnas.84.1.66
发表时间：
1987-01-01
影响因子：
11.1
作者：
HAWGOOD, S;BENSON, BJ;WHITE, RT
通讯作者：
WHITE, RT
合成磷脂与纯化的肺表面活性剂 6000 道尔顿脱辅基蛋白结合的生物物理活性。
影响因子：
3.4
作者：
Notter,RH;Shapiro,DL;Ohning,B;Whitsett,JA
通讯作者：
Whitsett,JA