Induction of biologically active interleukin-8 from lung epithelial cells by Burkholderia (Pseudomonas) cepacia products
Induction of biologically active interleukin-8 from lung epithelial cells by Burkholderia (Pseudomonas) cepacia products
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DOI：
10.1128/iai.65.2.617-622.1997
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发表时间：
1997-02-01
影响因子：
3.1
通讯作者：
Smith, AW
中科院分区：
文献类型：
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作者：
Palfreyman, RW;Watson, ML;Smith, AW
从囊性纤维化（CF）患者的痰液中分离出洋葱伯克霍尔德菌的频率正在增加，利用人A549肺上皮细胞系，我们研究了洋葱伯克霍尔德菌外产物刺激白细胞介素-8 （IL-8）释放的能力，从CF临床、非CF临床和非临床分离的洋葱伯克霍尔德菌中发现无细胞上清可以刺激IL-8的释放，水平从11.8 +/- 2.8到80.0 +/- 3.5 ng/ml不等。在白细胞介素-8 mRNA水平上也发现了类似的模式，通过检测白细胞介素-8对中性粒细胞胞内游离钙的影响和中和性抗白细胞介素-8抗体的抑制作用，证实了白细胞介素-8的生物活性，能够刺激单核细胞释放白细胞介素-8，但不能从A549细胞中释放白细胞介素-8，此外，细菌无细胞上清的刺激能力没有被多粘菌素B降低，但被煮沸明显降低。似乎与n -酰基高丝氨酸内酯无关。洋葱芽孢杆菌诱导上皮细胞释放IL-8的能力在CF的病理中可能是重要的。
The frequency of isolation of Burkholderia cepacia from the sputum of cystic fibrosis (CF) patients is increasing, Using the human A549 lung epithelial cell line, we have investigated the ability of B. cepacia exoproducts to stimulate interleukin-8 (IL-8) release, Cell-free supernatants from a panel of CF clinical, non-CF clinical, and nonclinical B. cepacia isolates were found to stimulate IL-8 release, with levels ranging from 11.8 +/- 2.8 to 80.0 +/- 3.5 ng/ml, A similar pattern was seen at the level of the IL-8 mRNA, The bioactivity of the IL-8 was confirmed by examining its effect on the intracellular free calcium in neutrophils and inhibition by a neutralizing anti-IL-8 antibody, B. cepacia lipopolysaccharide, which was able to stimulate IL-8 release from monocytes, did not release IL-8 from the A549 cells, Furthermore, the stimulating ability of the bacterial cell-free supernatant was not diminished by polymyxin B, was markedly reduced by boiling, and appeared unrelated to N-acylhomoserine lactones. The ability of B. cepacia to elicit IL-8 release from epithelial cells may be important in the pathology of CF.