Selectivity improvement in protein nanopatterning with a hydroxy-terminated self-assembled monolayer template
Selectivity improvement in protein nanopatterning with a hydroxy-terminated self-assembled monolayer template
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DOI：
10.1088/0957-4484/18/30/305304
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发表时间：
2007-08-01
期刊：
影响因子：
3.5
通讯作者：
Ohdomari, Iwao
中科院分区：
文献类型：
--
作者：
Miyake, Takeo;Tanii, Takashi;Ohdomari, Iwao
蛋白质纳米图技术，以满足减少非特异性吸附的要求。目的蛋白被选择性地固定在羟基端自组装的单层模板上，模板的模式被链霉亲和素作为中间分子修饰。作为中介分子的3-氨基丙基三甲氧基硅烷（APTES）由于其自身的非特异性吸附而诱导蛋白质的非特异性吸附。这些数据表明，减少靶蛋白和中间分子的非特异性吸附对提高蛋白质图谱的选择性很重要。作为改进的结果，一个直径为250纳米的蛋白质纳米图案已经被制造出来。
Protein nanopatterning techniques to fulfil the requirement for reducing nonspecific adsorption are demonstrated. A target protein is selectively immobilized on a hydroxy-terminated self-assembled monolayer template, the pattern of which is modified with streptavidin used as the intermediating molecule. 3-aminopropyltrimethoxysilane (APTES) used as the intermediating molecule induces nonspecific adsorption of proteins due to nonspecific adsorption of APTES itself. The data indicate that reducing nonspecific adsorption of both the target protein and the intermediating molecule is important for selectivity improvement in protein patterning. As a result of the refinement, a protein nanopattern of 250 nm in diameter has been fabricated.