Functional identification of the actual and potential stem cell compartments in mouse spermatogenesis
Functional identification of the actual and potential stem cell compartments in mouse spermatogenesis
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DOI：
10.1016/j.devcel.2007.01.002
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发表时间：
2007-02-01
影响因子：
11.8
通讯作者：
Yoshida, Shosei
中科院分区：
文献类型：
--
作者：
Nakagawa, Toshinori;Nabeshima, Yo-ichi;Yoshida, Shosei
为了阐明支持长寿命生物活跃循环组织持续存在的机制，我们通过脉冲追逐负责干细胞功能的未分化精原细胞，结合移植和脉冲标记后的再生试验，研究了小鼠生精干细胞系统的组成。我们证明，除了确实具有自我更新能力的“实际干细胞”之外，还存在第二个群体(“潜在干细胞”)，它能够自我更新，但在正常情况下不能自我更新。潜在的干细胞在正常的睾丸中迅速翻转，这表明它们属于转运放大的人群，而不是休眠的人群。在漫长的自然过程中，真正的干细胞偶尔会丢失，并被邻居的后代补偿。在这个过程中，潜在的干细胞被假设将其模式从转移扩增转变为自我更新，从而在确保精子发生的完整性方面发挥关键作用。
To clarify the mechanisms that support the continuity of actively cycling tissues of long-lived organisms, we investigated the composition of a mouse spermatogenic stem cell system by pulse-chase of the undifferentiated spermatogonia, the population responsible for stem cell functions, in combination with transplantation and regeneration assays after pulse-labeling. We demonstrate that in addition to "actual stem cells," which are indeed self-renewing, a second population ("potential stem cells") also exists, which is capable of self-renewing but do not self-renew in the normal situation. Potential stem cells rapidly turn over in normal testes, suggesting that they belong to the transit-amplifying, rather than the dormant, population. During the long natural course, actual stem cells are occasionally lost and compensated for by progeny of their neighbors. In this process, potential stem cells are postulated to shift their modes from transit amplification to self-renewal, thus playing an essential role to ensure spermatogenesis integrity.