Effects of lysosomotropic amines on human polymorphonuclear leucocyte function.
Effects of lysosomotropic amines on human polymorphonuclear leucocyte function.
复制标题
溶酶体胺对人多形核白细胞功能的影响。
作者：
C. Vandenbroucke;R. Thijssen;J. Marcelis;S. Sharma;J. Verhoef
溶体因子干扰溶酶体功能。我们研究了利多卡因，二苯胺和丹磺尸胺对人多形核白细胞（PMN）的几种功能的影响：酶释放，吞噬体-溶酶体融合，超氧阴离子产生与调理细菌刺激后，吞噬和杀死调理金黄色葡萄球菌。利多卡因抑制了所有测试的细胞功能。二苯胺可抑制中性粒细胞吞噬酶的释放和吞噬体-溶酶体的融合。这是伴随着超氧阴离子生成的增加。丹磺尸胺促进酶释放和吞噬体-溶酶体融合，并减少超氧阴离子的产生。这两种药物都不影响细菌吸收;细菌杀伤仅在丹磺尸胺处理的细胞中受损。尸胺，一种不能穿透细胞的类似物，对测试的任何功能都没有影响。
Lysosomotropic agents interfere with lysosome function. We studied the effects of the lysosomotropic amines: lidocaine, diphenylamine and dansylcadaverine on several functions of human polymorphonuclear leucocytes (PMN): enzyme release, phagosome-lysosome fusion, superoxide anion generation upon stimulation with opsonized bacteria, and phagocytosis and killing of opsonized Staphylococcus aureus. Lidocaine depressed all cellular functions tested. Diphenylamine reduced enzyme release and phagosome-lysosome fusion in phagocytosing PMN. This was accompanied by an increase in superoxide anion generation. Dansylcadaverine enhanced enzyme release and phagosome-lysosome fusion, and reduced superoxide anion generation. Neither of these two agents influenced bacterial uptake; bacterial killing was impaired only in dansylcadaverine treated cells. Cadaverine, an analogue that does not penetrate cells, had no effect on any of the functions tested.