Localized heat-shock induction in Drosophila melanogaster.
Localized heat-shock induction in Drosophila melanogaster.
复制标题
果蝇的局部热休克诱导。
DOI：
10.1002/jez.1402470312
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发表时间：
1988
期刊：
影响因子：
--
通讯作者：
M. Wolfner
中科院分区：
文献类型：
--
作者：
Scott A. Monsma;Robert L. Ard;John T. Lis;M. Wolfner
我们描述了一种诱导与热休克基因启动子融合的基因局部表达的技术。我们证明，通过用加热的针接触动物的某个区域，可以在细胞胚层形成后的任何发育阶段在果蝇中诱导局部热休克反应。诱导区域的大小可以通过改变温度和针尖大小等参数来改变。这里使用的测试系统是用果蝇 hsp26 基因和大肠杆菌 lacZ 基因融合体转化的黑腹果蝇菌株；通过对整个动物的β-半乳糖苷酶活性进行染色来监测该杂合基因的活性。诱导的 β-半乳糖苷酶活性仅限于加热区域的细胞；热激后48小时仍可检测到β-半乳糖苷酶活性。鉴于果蝇热休克启动子在异源系统中的热诱导性，我们建议该技术可用于在可以引入融合基因的任何生物体中空间控制地诱导感兴趣的基因。讨论了该技术在发育过程中跟踪细胞运动的其他用途。
We describe a technique for inducing localized expression of genes fused to heat-shock gene promoters. We demonstrate that a localized heat-shock response can be induced in Drosophila melanogaster at any developmental stage after formation of the cellular blastoderm by contacting a region of the animal with a heated needle. The size of the induced region can be altered by varying parameters such as the temperature and size of the needle tip. The test system utilized here is a D. melanogaster strain transformed with a fusion of the Drosophila hsp26 gene and the E. coli lacZ gene; the activity of this hybrid gene is monitored in whole animals by staining for beta-galactosidase activity. Induced beta-galactosidase activity is confined to the cells in the region of heating; the beta-galactosidase activity can still be detected 48 hr after the heat shock. Given the heat inducibility of Drosophila heat-shock promoters in heterologous systems, we suggest that this technique will be useful for allowing spatially controlled induction of a gene of interest in any organism into which fusion genes can be introduced. Additional uses of the technique for following cell movements during development are discussed.