Distribution of bone morphogenetic protein receptors in human scleral fibroblasts cultured in vitro and human sclera
Distribution of bone morphogenetic protein receptors in human scleral fibroblasts cultured in vitro and human sclera
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DOI：
10.3980/j.issn.2222-3959.2012.06.02
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发表时间：
2012-12-18
影响因子：
1.4
通讯作者：
Zeng, Jun-Wen
中科院分区：
文献类型：
--
作者：
Gao, Zhen-Ya;Huo, Li-Jun;Zeng, Jun-Wen
目的：目的：研究骨形态发生蛋白受体（BMPRs）在人巩膜成纤维细胞（HSFs）和巩膜中的分布。逆转录-聚合酶链反应（RT-PCR）检测HSFs中BMP-2和BMPRs的mRNA水平。免疫细胞荧光和western blot检测BMP-2和BMPRs在HSFs中的蛋白表达。结果：BMP-2和BMPRs在HSFs和人巩膜组织中不仅在mRNA水平表达，而且在蛋白水平表达。BMPRIA和BMPRII在体外培养的HSFs胞浆和细胞膜中的表达均高于BMPRIB，Western blot进一步证实了免疫细胞荧光的结果。结论：HSF中存在BMP-2及3种BMPR亚型，它们可能参与了巩膜的重塑。
AIM: To investigate the distribution of bone morphogenetic protein receptors (BMPRs) in human scleral fibroblsasts (HSFs) and in human sclera.METHODS: Primary HSFs were cultured in vitro. The mRNA levels of BMP-2 and BMPRs in HSFs were assayed by reverse transcription-polymerase chain reaction (RT-PCR). The protein distributions of BMP-2 and BMPRs in HSFs were further detected by immunocytofluorescence and western blot. Their protein expression was also detected in frozen human posterior scleral sections by immunohistofluorescence.RESULTS: BMP-2 and BMPRs were expressed in both HSFs and human sclera not only at mRNA level but also at protein level. The expressions of BMPRIA and BMPRII were higher than that of BMPRIB in the cytoplasm and cell membrane of HSFs in vitra Western blot further verified the results of immunocytofluorescence. In human sclera, BMP2, BMPR IB and BMPR II were found to be expressed in the cytomatrix of HSF, and weak signal was detected about BMPRIA.CONCLUSION: BMP-2 and all three subtypes of BMPRs were found in HSFs and may play a role in scleral remodeling.