Ultracytochemical localization of Na+,K+‐activated ATPase in chloride cells from the gills of a euryhaline teleost
Ultracytochemical localization of Na+,K+‐activated ATPase in chloride cells from the gills of a euryhaline teleost
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DOI：
10.1002/ar.1091930107
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发表时间：
1979-01
期刊：
影响因子：
--
通讯作者：
S. Hootman;C. W. Philpott
中科院分区：
文献类型：
--
作者：
S. Hootman;C. W. Philpott
将鱼从咸水转移到海水后，菱形针鱼鳃中电解质转运酶、钠、钾激活的三磷酸腺苷酶 (Na+,K+-ATPase) 的活性显着增加。在适应海水的针鱼鳃上皮中，Na+,K+-ATP酶通过其钾依赖性磷酸酶（K+-NPPase）活性进行细胞化学定位，表明氯细胞是该酶的主要位点。在细胞亚细胞中，观察到最重的反应产物沉积在吻合质膜小管迷宫的细胞质膜表面，该迷宫遍布氯细胞细胞质。还在隐窝顶膜的细胞质表面和隐窝下方的细胞质中的囊泡的细胞质表面上证明了酶活性。从细胞化学孵育介质中除去钾或加入 10 mM 哇巴因消除了与这些膜相关的反应产物。这些细胞化学结果的意义将参考当前氯细胞功能的假设进行讨论。
The activity of the electrolyte transport enzyme, sodium, potassium‐activated adenosine triphosphatase (Na+,K+‐ATPase), in the gills of the pinfish, Lagodon rhomboides, increased markedly following transfer of fish from brackish water to seawater. Cytochemical localization of Na+,K+‐ATPase via its potassium‐dependent phosphatase (K+‐NPPase) activity in the branchial epithelium of pinfish adapted to seawater demonstrated that chloride cells are the major sites for the enzyme. Subcellularly, the heaviest depositions of reaction product were observed lining the cytoplasmic membrane surfaces of the labyrinth of anastomosing plasma membrane tubules that ramifies throughout the chloride cell cytoplasm. Enzyme activity was demonstrated also on the cytoplasmic surface of the apical crypt membrane and on the cytoplasmic surfaces of vesicles in the cytoplasm subjacent to the crypt. Deletion of potassium from the cytochemical incubation medium or inclusion of 10 mM ouabain abolished the reaction products associated with these membranes. The significance of these cytochemical results is discussed with reference to current hypotheses of chloride cell function.