Vascular-associated lymphoid tissue (VALT) involvement in aortic aneurysm
Vascular-associated lymphoid tissue (VALT) involvement in aortic aneurysm
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DOI：
10.1016/s0021-9150(00)00441-x
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发表时间：
2001-01-01
期刊：
影响因子：
5.3
通讯作者：
Lord, RSA
中科院分区：
文献类型：
--
作者：
Bobryshev, YV;Lord, RSA
血管相关淋巴组织（VALT）是由免疫活性细胞和抗原提呈细胞聚集而成的，最近在动脉壁中被发现。在这项研究中，我们调查了腹主动脉瘤（AAA）的免疫反应中的VALT的参与。在手术过程中从31名患有动脉粥样硬化性肾下腹主动脉瘤的患者中收集组织样本，直径范围为5至8 cm。立即冷冻标本，并使用单和双免疫组化染色进行检查。使用细胞类型特异性抗体鉴定T细胞亚群、B细胞、树突细胞和巨噬细胞。通过电子显微镜检查细胞接触。大部分炎性浸润位于外膜。T细胞是大多数炎性浸润的主要细胞类型，但在17例病例中，还观察到典型的淋巴滤泡和B细胞形成的生发中心。在8例中，淋巴滤泡聚集在淋巴结样结构。树突状细胞存在于所有炎性浸润和接触淋巴细胞。目前的观察表明，在主动脉瘤中，VALT参与免疫反应，其激活主要发生在外膜。外膜中淋巴滤泡和淋巴结样结构的形成表明，VALT可能局部服务于血管壁中细胞和体液免疫反应的整个复合体。(C)2001爱思唯尔科学爱尔兰有限公司保留所有权利。
Vascular-associated lymphoid tissue (VALT) consisting of accumulations of immunocompetent and antigen presenting cells has recently been recognised in the arterial wall. In this study, we investigated the involvement of VALT in immune responses in abdominal aortic aneurysms (AAAs). Tissue samples were collected during operations from 31 patients with atherosclerotic infrarenal abdominal aortic aneurysms ranging in diameters from 5 to 8 cm. The specimens were immediately frozen and examined using single and double immunohistochemical staining. T-cell subpopulations, B-cells, dendritic cells and macrophages were identified using cell type specific antibodies. Cell contacts were examined by electron microscopy. Most inflammatory infiltrates were found in the adventitia. T-cells were the predominant cell type in a majority of inflammatory infiltrates but in seventeen cases, typical lymphoid follicles with B-cells forming germinative centres were also observed. In eight cases, the lymphoid follicles aggregated in lymph node-like structures. Dendritic cells were present within all inflammatory infiltrates and contacted lymphocytes. The present observations show that in aortic aneurysm, VALT is involved in immune responses and its activation mostly occurs in the adventitia. The formation of lymphoid follicles and lymph node-like structures in the adventitia suggests that VALT might locally serve the entire complex of both cellular and humoral immune responses in the aneurysmal wall. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.