Panax Notoginseng Saponins Combined with Dual Antiplatelet Drugs Potentiates Anti-Thrombotic Effect with Alleviated Gastric Injury in A Carotid Artery Thrombosis Rat Model.
Panax Notoginseng Saponins Combined with Dual Antiplatelet Drugs Potentiates Anti-Thrombotic Effect with Alleviated Gastric Injury in A Carotid Artery Thrombosis Rat Model.
复制标题
DOI：
10.1016/j.jstrokecerebrovasdis.2022.106597
复制
发表时间：
2022-06
期刊：
影响因子：
--
通讯作者：
Yanyan Wu;Wenting Wang;N. Kou;Ming-ming Wang;Lin Yang;Yul Miao;Ziwei Tang;Yimeng Gu;Yan Ma;Mei Xue;D. Shi
中科院分区：
文献类型：
--
作者：
Yanyan Wu;Wenting Wang;N. Kou;Ming-ming Wang;Lin Yang;Yul Miao;Ziwei Tang;Yimeng Gu;Yan Ma;Mei Xue;D. Shi
目的观察三七总皂苷（PNS）与双重抗血小板药物（DAPT）联合应用的抗血小板作用，并通过环氧合酶/前列腺素途径探讨其作用机制。麻醉后在体解剖显微镜下观察软脑膜微循环。测量颈动脉血栓湿重。苏木精-伊红染色观察胃粘膜损伤情况。二磷酸腺苷诱导比浊法检测血小板聚集率。流式细胞术检测血小板CD 62 p表达。结果三七总皂苷和当归总皂苷均能增加模型大鼠软脑膜血流量，降低模型大鼠红细胞聚集性和白细胞粘附性，降低血浆血栓素B2、6-酮前列腺素F1 α、组织型纤溶酶原激活物、纤溶酶原激活物抑制剂和纤维蛋白片段D的含量。与DAPT相比，PNS和DAPT进一步降低了颈动脉血栓形成的重量，增强了对血小板聚集的抑制，增加了组织纤溶酶原激活物水平，降低了纤维蛋白片段D水平。结论PNS联合DAPT可增强DAPT的抗血栓作用，减轻DAPT引起的胃损伤。其机制可能与增强抗血小板聚集和激活纤溶系统以及上调胃粘膜6-酮前列腺素F1 α表达有关。
ObjectiveTo observe the combination effects of Panax notoginseng saponins (PNS)and dual antiplatelet drugs (DAPT), and to explore the mechanism via cyclooxygenase /prostaglandin pathway.MethodsRight carotid artery thrombosis was induced in Wistar rats by infiltration with 70% FeCl3, and the animals were randomly divided into sham group, model group, DAPT group and PNS + DAPT group, intragastrically treated for 4 weeks. The cerebral pia mater microcirculation was observed in vivo after anesthetizing by anatomical microscope. The wet weight of carotid artery thrombosis was measured. Gastric mucosal injury was observed by hematoxylin and eosin staining. Platelet aggregation rate was detected with adenosine diphosphate -induced turbidimetry. Platelet CD62p expression was detected by flow cytometry. Concentrations of 6-Ketoprostaglandin F1 alpha, prostaglandin E2in gastric mucosa and thromboxane B2, 6-Ketoprostaglandin F1 alpha, tissue plasminogen activator, plasminogen activator inhibitor, and fibrin fragment D in the plasma were measured by radioimmunoassay.ResultsPNS and DAPT increased the blood flow volume of cerebral pia mater and decreased erythrocyte aggregation and leukocyte adhesion of model rats. Compared to DAPT, PNS and DAPT further reduced the weight of carotid artery thrombosis with enhanced inhibition of platelet aggregation, increased tissue plasminogen activator levels and decreased fibrin fragment D levels. PNS and DAPT alleviated gastric injury induced by dual antiplatelet drugs and upregulated the expression of 6-Ketoprostaglandin F1 alpha in the gastric mucosa compared with DAPT.ConclusionsPNS combined with DAPT increased anti-thrombosis effects of DAPT and mitigated DAPT-related gastric injury. The underlying mechanisms may be associated with enhanced antiplatelet aggregation and activation of the fibrinolytic system and up-regulation of 6-Ketoprostaglandin F1 alpha expression in gastric mucosa.