Chitosan/hydroxyapatite hybrid scaffold for bone tissue engineering
Chitosan/hydroxyapatite hybrid scaffold for bone tissue engineering
复制标题
DOI：
10.3233/bme-140975
复制
发表时间：
2014-01-01
影响因子：
1
通讯作者：
Velard, F.
中科院分区：
文献类型：
--
作者：
Brun, V.;Guillaume, C.;Velard, F.
背景：为了促进严重骨缺损后的再生，目前使用自体骨移植物和生物材料的组合。这种技术的主要缺点仍然是自体材料和第二手术部位的可用性，引起疼痛和发病率。目的：我们的目的是研究三维杂化生物降解支架的体外生物相容性，结合羟基磷灰石的骨传导特性和壳聚糖的抗炎特性。通过显微镜观察、平衡溶胀率和随时间推移的重量损失测量来表征杂化支架。使用培养7、14和21天的原代人骨细胞进行体外研究。通过碱性磷酸酶（ALP）活性测定，细胞的活力，增殖，形态和分化进行了评估。结果：表征我们的支架表现出多孔性，亲水性和可生物降解的特性。在体外研究表明，这些支架诱导细胞死亡和增殖略有下降相比，培养塑料基板控制conditions. CONCLUSIONS，以及增加短期骨诱导properties.结论：在这项研究中，我们提供了证据，我们的混合羟基磷灰石/壳聚糖支架可以适用于骨填充。
BACKGROUND: To favor regeneration following critical bone defect, a combination of autologous bone graft and biomaterials is currently used. Major drawbacks of such techniques remain the availability of the autologous material and the second surgical site, inducing pain and morbidity.OBJECTIVE: Our aim was to investigate the biocompatibility in vitro of three dimensions hybrid biodegradable scaffolds combining osteoconductive properties of hydroxyapatite and anti-inflammatory properties of chitosan.METHODS: Hybrid scaffolds were characterized by microscopic observations, equilibrium swelling ratio and overtime weight loss measurements. In vitro studies were performed using primary human bone cells cultured for 7, 14 and 21 days. Cell viability, proliferation, morphology and differentiation through alkaline phosphatase (ALP) activity measurement were assessed.RESULTS: Characterization of our scaffolds demonstrated porous, hydrophilic and biodegradable characteristics. In vitro studies showed that these scaffolds have induced slight decrease in cell death and proliferation comparing to the culture plastic substrate control condition, as well as increased short term osteoinductive properties.CONCLUSIONS: In this study, we have provided evidence that our hybrid hydroxyapatite/chitosan scaffolds could be suitable for bone filling.