Thermally assisted fast atom bombardment: a new approach toward optimization of analyses by fast atom bombardment mass spectrometry.
Thermally assisted fast atom bombardment: a new approach toward optimization of analyses by fast atom bombardment mass spectrometry.
复制标题
热辅助快原子轰击：快原子轰击质谱分析优化的新方法。
DOI：
10.1021/ac00290a051
复制
发表时间：
1985
影响因子：
7.4
通讯作者：
Holland,JF
中科院分区：
文献类型：
--
作者：
Ackermann,BL;Watson,JT;Holland,JF
本文研究了饱和溶液在热辅助快原子轰击（TA-FAB）质谱基体制备中的应用。某些饱和基质在加热时模拟甘油的所需性质，而不会产生太多不必要的背景。此外，由于FAB期间探针尖端的受控加热，经常观察到分析物和基质离子的解吸曲线的差异。在有利的情况下，可以收集合适的数据用于有效的背景减除，这在常规FAB条件下是不可用的。使用糖基质（如葡萄糖）的TA-FAB的初步结果与使用甘油的常规FAB分析相比更有利。本文介绍了用TA-FAB分析几种非挥发性生物分子的结果，结果表明，用这种方法可以得到亚微图范围内的检测限，特别是当发现一种物理化学上与所研究的分析物相适合的基质时。一组被统称为解吸电离（DI）法的质谱技术大大拓宽了质谱分析的范围，特别是分析高分子量或低挥发性分子的能力。DI方法通常涉及测量源自安装在离子源室内的表面的离子。DI方法不同于传统的电离模式，如电子碰撞（El）和化学电离（Cl），因为将样品引入气相不是电离的先决条件。虽然各种DI方法的电离方法不同，但每个过程都是在解吸/电离之前以冷凝状态的分析物开始的。在Beckey于1969年（1）引入场解吸（FD）之后，许多其他DI技术也被开发出来。这些方法的例子包括：等离子体解吸（PD）（2）、二次离子质谱（西姆斯）（3）和激光解吸（LD）（4）。最近，巴伯及其同事在1981年引入了一种称为快速原子轰击（FAB）的技术（5）。该方法利用高能原子束（6-10 keV）来实现溶解在粘性低蒸气压基质中的非挥发性分析物的解吸/电离。虽然许多基质已被用于不同的成功，最常用的基质是甘油。FAB今天的广泛使用主要是由于其低成本和仪器简单，因此比其他DI方法更具有商业实用性。
The use of saturated solutions in the preparation of matrices for thermally assisted fast atom bombardment (TA-FAB) mass spectrometry has been Investigated. Certain saturated ma-trices, when heated, simulate the desired properties of glycerol without creating as much unwanted background. In addition, differences In the desorption profiles for analyte and matrix Ions are often observed as a result of controlled heatlpg of the probe tip during FAB. In favorable cases, suitable data can be collected for valid background subtraction, which under conventional FAB conditions are not available. Initial results with TA-FAB using saccharide ma-trices, such as glucose, compare favorably with conventional FAB analyses using glycerol. Results are presented for the TA-FAB analysis of several nonvolatile biomolecules which Indicate that detection limits In the submlcrogram range can be obtained by this method, particularly when a matrix Is found which Is physlochemically well-suited to the analyte being studied.A group of mass spectrometric techniques known collec-tively as desorption-ionization (DI) methodshave significantly broadened the scope of mass spectrometric analysis, particularly in the capacity to analyze molecules of either high molecular weight or low volatility. DI methods typically involve the measurement of ions originating from a surface mounted within the ion source chamber. DI methods differ from conventional ionization modes, such as electron impact (El) and chemical ionization (Cl), in that introduction of the sample into the gas phase is not a prerequisite for ionization. While the approachto ionization differs among the various DI methodologies, each process begins with the analyte in the condensed state prior to desorption/ionization. Following the introduction of field desorption (FD) byBeckey in 1969 (1), many other DI techniques have been developed. Examples of these include: plasmadesorption (PD)(2), secondary ion mass spectrometry (SIMS)(3), and laser desorption (LD)(4). More recently, a technique known as fast atom bombardment (FAB) was introduced by Barber and co-workers in 1981 (5). This method utilizes an energetic beam of atoms (6-10 keV) to effect desorption/ionization of nonvolatile analytes dissolved in a viscous, low vapor pressure matrix. While many matrices have been used with varying success, the most frequently chosen matrix is glycerol. The widespread use of FAB today is largely due to its low cost and instrumental simplicity, therefore leading to greater commercial availability than other DI methods.